Concept
Protein production
Related publications (160)
Horner-Wadsworth-Emmons olefination for bioconjugation
Through site-specific generation of intermediary reactive aldehydes, Horner-Wadsworth-Emmons olefination can now deliver selective functionalization of stable recombinant proteins and monoclonal antibodies, whilst preserving protein integrity. ...
Springernature2024Living Photovoltaics based on Recombinant Expression of MtrA Decaheme in Photosynthetic Bacteria
Ardemis Anoush Boghossian, Melania Reggente, Mohammed Mouhib, Alessandra Antonucci, Nils Schürgers, Sara Politi
At the center of microbial bioelectricity applications lies the critical need to express foreign heme proteins that are capable of redirecting the electron flux of the cell’s metabolism. This study presents bioengineered Synechocystis sp. PCC 6803 cells ca ...
2023Rational Design of Chemically Controlled Antibodies and Protein Therapeutics
Li Tang, Bruno Emanuel Ferreira De Sousa Correia, Sandrine Madeleine Suzanne Georgeon, Pablo Gainza Cirauqui, Anthony Marchand, Leo Scheller, Lucia Bonati, Stéphane Rosset, Sailan Shui
Protein-based therapeutics, such as monoclonal antibodies and cytokines, are important therapies for various pathophysiological conditions such as oncology, autoimmune disorders, and viral infections. However, the wide application of such protein therapeut ...
2023Improved Cell-Free Transcription-Translation Reactions in Microfluidic Chemostats Augmented with Hydrogel Membranes for Continuous Small Molecule Dialysis
Sebastian Maerkl, Laura Sophie Grasemann, Barbora Lavickova
Increasing the protein production capacity of the PURE cell-free transcription-translation (TX-TL) system will be key to implementing complex synthetic biological circuits, and to establishing a fully self-regenerating system as a basis for the development ...
AMER CHEMICAL SOC2022Computational design of novel protein–protein interactions – An overview on methodological approaches and applications
Bruno Emanuel Ferreira De Sousa Correia, Anthony Marchand, Alexandra Krina Van Hall-Beauvais
Protein–protein interactions (PPIs) govern numerous cellular functions in terms of signaling, transport, defense and many others. Designing novel PPIs poses a fundamental challenge to our understanding of molecular interactions. The capability to robustly ...
2022Microfluidics and cell-free synthetic biology in the development of new diagnostic and therapeutic platforms
In phenylketonuria, absence or malfunction of the phenylalanine hydroxylase enzyme results in toxic accumulation of phenylalanine in the body. An injectable recombinant enzyme therapy was recently approved and has the potential to improve the quality of li ...
EPFL2021MAS NMR Studies of Hierarchical Interplay in Protein Dynamics
The functionality of proteins is governed by the interplay between their structure and dynamics. Thus, understanding the mechanism and nature of protein motion is essential to understand their biological activity. Here we present and evaluate a novel metho ...
EPFL2021Overexpression of transcription factor Foxa1 and target genes remediate therapeutic protein production bottlenecks in Chinese hamster ovary cells
Nicolas Mermod, Iris Bodenmann
Despite extensive research conducted to increase protein production from Chinese hamster ovary (CHO) cells, cellular bottlenecks often remain, hindering high yields. In this study, a transcriptomic analysis led to the identification of 32 genes that are co ...
WILEY2020Site-Specific Protein Conjugation onto Fluorescent Single-Walled Carbon Nanotubes
Ardemis Anoush Boghossian, Shang-Jung Wu, Vitalijs Zubkovs
Semiconducting single-walled carbon nanotubes (SWCNTs) are among the few photostable optical emitters that are ideal for sensing, imaging, drug delivery, and monitoring of protein activity. These applications often require strategies for immobilizing prote ...
2020Transient vitamin B5 starving improves mammalian cell homeostasis and protein production
Maintaining a metabolic steady state is essential for an organism's fitness and survival when confronted with environmental stress, and metabolic imbalance can be reversed by exposing the organism to fasting. Here, we attempted to apply this physiological ...
ACADEMIC PRESS INC ELSEVIER SCIENCE2020