Concept

Fluorescence in situ hybridization

Related publications (222)

Establishment of recombinant mammalian cell lines

Recombinant proteins are gaining in importance for therapeutic applications. The proteins are expressed in stable cell lines, within which recombinant DNA has incorporated into the host cells genome. Identification and isolation of extremely high producers ...

EPFL2005

'Label-free' DNA analysis using a-Si:H UV sensors

Giovanni De Cesare, Carlotta Guiducci

In this work we present the first results on a 'label-free' DNA analysis system based on hydrogenated amorphous silicon (a-Si:H) thin film sensors. The detection mechanism is based on absorbance measurements performed on DNA molecules in a buffer solution. ...

2005

Nanofabrication of protein-patterned substrates for future cell adhesion experiments

Nico de Rooij, Laure Aeschimann

A method for fabricating sub-micrometer size adhesion sites for future experiments in cell biology is presented. Glass substrates were coated with a thin layer of InSnO and SiO2. The SiO2 was structured by means of electron beam lithography and reactive io ...

2005

The quenching of electrochemiluminescence upon oligonucleotide hybridization

Nico de Rooij

Many genomic assays rely on a distance-dependent interaction between luminescent labels, such as luminescence quenching or resonance energy transfer. We studied the interaction between electrochemically excited Ru(bpy) 3 2+ and Cy5 in a hybridization assay ...

2004

Labeling of fusion proteins with synthetic fluorophores in live cells

Horst Vogel, Kai Johnsson, Horst Pick, Claudio Arrivoli, Antje Keppler

A general approach for the sequential labeling of fusion proteins of O6-alkylguanine-DNA alkyltransferase (AGT) with different fluorophores in mammalian cells is presented. AGT fusion proteins with different localizations in the cell can be labeled specifi ...

2004

Solvation dynamics at water-ZrO2 interfaces

Majed Chergui, Jacques-Edouard Moser, Frank van Mourik, Erwin Portuondo Campa

The dye coumarin 343 (C343) adsorbed on the surface of ZrO2 nanoparticles in aq. solns. was used to study the solvation dynamics close to these surfaces. The time-resolved Stokes shift was measured by femtosecond frequency-resolved upconversion. The absorp ...

2004

Femtosecond-irradiation-induced refractive-index changes and channel waveguiding in bulk Ti3+: Sapphire

René Salathé, Christian Depeursinge, Tristan Colomb

We have employed femtosecond laser writing in order to induce refractive- index changes and waveguides in Ti3+-doped sapphire. Doping the sapphire crystal with an appropriate ion significantly reduces the threshold for creating structural changes, thus e ...

2004

Femtosecond-irradiation-induced refractive-index changes and channel waveguiding in bulk Ti3+: Sapphire

René Salathé, Christian Depeursinge, Tristan Colomb

We have employed femtosecond laser writing in order to induce refractive-index changes and waveguides in Ti3+-doped sapphire. Doping the sapphire crystal with an appropriate ion significantly reduces the threshold for creating structural changes, thus enab ...

2004

DNA detection by integrable electronics

Carlotta Guiducci, Giampaolo Zuccheri

This paper presents a new electronic methodology to detect DNA hybridization for rapid identification of diseases, as well as food and environmental monitoring on a genetic base. The proposed solution exploits a new (electrical) capacitive measurement circ ...

2004

Parallel Confocal Detection of Single Biomolecules using Diffractive Optics and Integrated Detector Units

The past few years we have witnessed a tremendous surge of interest in so-called array-based miniaturised analytical systems due to their value as extremely powerful tools for high-throughput sequence analysis, drug discovery and development, and diagnosti ...

2004

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