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The kinome of the human malaria parasite Plasmodium falciparum comprises representatives of most eukaryotic protein kinase groups, including kinases which regulate proliferation and differentiation processes. Despite extensive research on most plasmodial e ...
We present a microfluidic device for on-chip analysis of low-concentration protein biomarkers. A new detection method, based on the magnetic capture of proteins via superparamagnetic beads and counting the surface coverage of the latter is presented. The p ...
The formation of crossovers is a fundamental genetic process. The XPF-family endonuclease Mus81-Mms4 (Eme1) contributes significantly to crossing over in eukaryotes. A key question is whether Mus81-Mms4 can process Holliday junctions that contain four unin ...
A bubble cell capillary classically used to extend the optical path length for UV-vis detection is employed here to trap magnetic beads. With this system, a large amount of beads can be captured without inducing a strong pressure drop, as it is the case wi ...
We review new and established methods for the chemical modification of proteins in living cells and highlight recent applications. The review focuses on tag-mediated protein labeling methods, such as the tetracysteine tag and SNAP-tag, and new developments ...
We present the rapid fabrication of custom protein micro-arrays, in less than 35 minutes, on the surface of immobilized beads using microchannel-guided flows driven by a single syringe pump. The commercially available beads provide robust micro-array surfa ...
We present techniques and methodologies for the manipulation of magnetic micro- and nanoparticles ('beads') in microfluidic systems. We first introduce the most important forces that act on magnetic particles in a microfluidic system. Starting with the mag ...
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Polymer brushes represent an interesting platform for the development of high-capacity protein binding surfaces. Whereas the protein binding properties of polymer brushes have been investigated before, this manuscript evaluates the feasibility of poly(glyc ...
Proteomic studies require analytical methods capable of separating, identifying and quantifying thousands of proteins. Consequently, developing separation methodologies with high resolving power necessary to separate complex protein samples prior to protei ...
Chromatin immunoprecipitation experiments followed by ultra-high-throughput sequencing (ChIP-seq) is becoming the method of choice to identify transcription factor binding sites in prokaryotes and eukaryotes in vivo. Here, we review the computational steps ...