Concept
Microscopy
Related publications (1,000)
Surface passivation and functionalisation for mass photometry
Suliana Manley, Jenny Sülzle, Laila Abdelaziz Abdelmoniem Elfeky
Interferometric scattering (iSCAT) microscopy enables the label-free observation of biomolecules. Consequently, single-particle imaging and tracking with the iSCAT-based method known as mass photometry (MP) is a growing area of study. However, establishing ...
Wiley2024Compact and effective photon-resolved image scanning microscope
Fluorescence confocal laser-scanning microscopy (LSM) is one of the most popular tools for life science research. This popularity is expected to grow thanks to single-photon array detectors tailored for LSM. These detectors offer unique single-photon spati ...
Spie-Soc Photo-Optical Instrumentation Engineers2024Label-Free Techniques for Probing Biomolecular Condensates
Aleksandra Radenovic, Wayne Yang Wen Wei, Khalid Ashraf Mohie Ibrahim, Helena Miljkovic, Akhil Sai Naidu
Biomolecular condensates play important roles in a wide array of fundamental biological processes, such as cellular compartmentalization, cellular regulation, and other biochemical reactions. Since their discovery and first observations, an extensive and e ...
Amer Chemical Soc2024The multimodality cell segmentation challenge: toward universal solutions
Sahand Jamal Rahi, Vojislav Gligorovski, Marco Labagnara, Jun Ma, Xin Yang, Maxime Emmanuel Scheder, Yao Zhang, Bo Wang, Yixin Wang, Lin Han
Cell segmentation is a critical step for quantitative single-cell analysis in microscopy images. Existing cell segmentation methods are often tailored to specific modalities or require manual interventions to specify hyper-parameters in different experimen ...
Nature Portfolio2024Advances in High-Speed, Multiparametric Atomic Force Microscopy
After decades of technological advancements, high-speed atomic force microscopy (HS-AFM) has emerged as a powerful technique for visualizing dynamic processes. At the nanoscale, the AFM provides valuable insights into the sample by sensing minute interacti ...
EPFL2024Live-cell imaging powered by computation
The proliferation of microscopy methods for live-cell imaging offers many new possibilities for users but can also be challenging to navigate. The prevailing challenge in live-cell fluorescence microscopy is capturing intra-cellular dynamics while preservi ...
Berlin2024Subwavelength imaging using a solid-immersion diffractive optical processor
Demetri Psaltis, Carlo Gigli, Niyazi Ulas Dinç, Yang Li
Phase imaging is widely used in biomedical imaging, sensing, and material characterization, among other fields. However, direct imaging of phase objects with subwavelength resolution remains a challenge. Here, we demonstrate subwavelength imaging of phase ...
Springernature2024Subsurface fluorescence time-of-flight imaging using a large-format single-photon avalanche diode sensor for tumor depth assessment
Edoardo Charbon, Claudio Bruschini, Arin Can Ülkü, Yichen Feng
Significance: Fluorescence guidance is used clinically by surgeons to visualize anatomical and/or physiological phenomena in the surgical field that are difficult or impossible to detect by the naked eye. Such phenomena include tissue perfusion or molecula ...
Spie-Soc Photo-Optical Instrumentation Engineers2024Revealing the Mechanism Underlying 3D-AFM Imaging of Suspended Structures by Experiments and Simulations
The invention of 3D atomic force microscopy (3D-AFM) has enabled visualizing subnanoscale 3D hydration structures. Meanwhile, its applications to imaging flexible molecular chains have started to be experimentally explored. However, the validity and princi ...
Wiley-V C H Verlag Gmbh2024Fluorescence microscopy: A statistics-optics perspective
Fundamental properties of light unavoidably impose features on images collected using fluorescence microscopes. Accounting for these features is often critical in quantitatively interpreting microscopy images, especially those gathering information at scal ...
Amer Physical Soc2024