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A PEG-based polymer matrix is developed within which biol. macromols. and living mammalian cells could be easily dispersed. Constraints placed upon such a matrix by a desire that it be generally processable in the presence of biol. macromols. and living ce ...
For large-scale applications in biotechnology, cultivation of mammalian cells in suspension is an essential prerequisite. Typically, suspension cultures are grown in glass spinner flasks filled to less than 50% of the nominal volume. We propose a superior ...
Adult female mice of the "sensitive" NMRI/Han strain ovulate diploid oocytes after gonadotropin treatment. Other mouse strains are "non-sensitive" with respect to the ovulation of such diploid oocytes. In this study we combined the impaired ovarian situati ...
The ovulation of diploid oocytes, abnormally arrested at or during the first meiotic division, is an inheritable trait (DiplI) in mice and modulated by a maternally transmitted factor. By repeated backcrossing, mouse strains with identical nuclear encoded ...
The centrosome comprises a pair of centrioles and associated pericentriolar material, and it is the principal microtubule-organizing centre of most animal cells. Like the genetic material, the centrosome is duplicated once and only once during the cell cyc ...
A significant number of diploid oocytes is ovulated from adult NMRI/Han mice treated with high doses of gonadotrophins. This inhibition of the first meiotic division is very likely caused by an altered communication between the germ cell and the surroundin ...
The centrosome is the principal microtubule organizing center (MTOC) of animal cells. Accurate centrosome duplication is fundamental for genome integrity and entails the formation of one procentriole next to each existing centriole, once per cell cycle. Th ...
In both animal cells and fungi, cytokinesis proceeds via a contractile actomyosin ring (CAR). Many CAR components and regulators are evolutionarily conserved. In Schizosaccharomyces pombe, the spatial cue for cytokinesis is provided by Mid1p/Anillin, where ...
Formation of the microtubule-based centriole is a poorly understood process that is crucial for duplication of the centrosome, the principal microtubule-organizing center of animal cells . Five proteins have been identified as being essential for centriole ...
Animal cell (Chinese Hamster Ovary) concn. was detd. online in a packed bed process using dielec. spectroscopy. This enabled the evaluation of the effect of temp. on specific metabolic rates during 3 mo of continuous culture. The effect of low cultivation ...
