Concept

Organelle

Related publications (179)

Mutants of human O6-alkylguanine-DNA alkyltransferase with improved properties

Kai Johnsson, Christian Heinis, Alexandre Juillerat, Christopher Chidley, Thomas Gronemeyer, Antje Keppler

The invention relates to human O6-alkylguanine-DNA alkyltransferase (hAGT) mutants showing, when compared to the wild type human AGT, two or more advantageous properties selected from (a) reduced DNA interaction; (b) localization of the expressed protein i ...

(EPFL Ecole Polytechnique Federale de Lausanne, Switz.).2005

Centrosomes: hooked on the nucleus

Pierre Gönczy

A recent study has shown that the Hook protein ZYG-12 of Caenorhabditis elegans is essential for attachment of the centrosome to the nucleus. The results suggest a novel mechanism for the tight coupling between these two organelles, and shed new light on i ...

2004

Positioning and electrophysiological characterization of individual cells and reconstituted membrane systems on microstructured carriers

Horst Vogel

The invention relates to a measuring device which permits a very simple positioning of cells and vesicles respective of cell membranes on planar carriers. The invention also relates to a corresponding highly efficient method for the positioning and electri ...

2004

A method for introducing a nucleic acid into a eukaryotic cell

Florian Maria Wurm, Martin Jordan, Philippe Pierre Girard

Described is method for introducing a desired nucleic acid into a eukaryotic host cell, said method comprising a) admixing the desired nucleic acid, a mono- and/or divalent metal cation and a eukaryotic host cell lacking a cell wallb) incubating the mixtur ...

2003

Multiaperture sample positioning and analysis system

Horst Vogel

Systems for positioning and/or analyzing samples such as cells, vesicles, cellular organelles, and fragments, derivs., and mixts. thereof, for elec. and/or optical anal., esp. relating to the presence and/or activity of ion channels. [on SciFinder (R)] ...

(Switz.).2003

Covalent labeling of fusion proteins with chemical probes in living cells

Horst Vogel, Kai Johnsson, Horst Pick, Alexandre Juillerat, Thomas Gronemeyer, Antje Keppler

A general method for the specific and covalent labeling of fusion proteins in vivo is described. The approach is based on the unusual mechanism of the human DNA repair protein O6-alkylguanine-DNA alkyl-transferase, which irreversibly transfers an alkyl gro ...

2003

Eukaryotic cells and microbial pathogens: a familiar couple take centre stage

Françoise Gisou van der Goot Grunberg

The interaction of a microbial pathogen with its host is a highly dynamic process shaped by evolution. Understanding cellular responses to microbial products is essential for a complete understanding of virulence. Similarly, studies aimed at determining ho ...

Nature Publishing Group2003

Structure-based thermodynamic analysis of caspases reveals key residues for dimerization and activity

Ursula Röthlisberger

Cysteine-dependent aspartic proteases (caspases) are a family of enzymes which play a crucial role in apoptosis. Caspases accumulate in eukaryotic cells in the form of low-activity proenzyme precursors. Proteolytic cleavage of specific sites triggers confo ...

2003

Compositional Heterogeneity Reflects Partial Dehydration in Three-dimensional Crystals of Bacteriorhodopsin

Majed Chergui, Erwin Portuondo Campa, Goran Zgrablic, Stefan Haacke, Selma Schenkl

Absorption, fluorescence and excitation spectra of three-dimensional bacteriorhodopsin crystals harvested from a lipidic cubic phase are presented. The combination of the spectroscopic expts. performed at room temp., controlled pH and full external hydrati ...

2003

Progress in the analysis of membrane protein structure and function

Henning Paul-Julius Stahlberg, Andreas Engel

Structural information on membrane proteins is sparse, yet they represent an important class of proteins that is encoded by about 30% of all genes. Progress has primarily been achieved with bacterial proteins, but efforts to solve the structure of eukaryot ...

Wiley-Blackwell2002

Graph Chatbot

Chat with Graph Search

Ask any question about EPFL courses, lectures, exercises, research, news, etc. or try the example questions below.

DISCLAIMER: The Graph Chatbot is not programmed to provide explicit or categorical answers to your questions. Rather, it transforms your questions into API requests that are distributed across the various IT services officially administered by EPFL. Its purpose is solely to collect and recommend relevant references to content that you can explore to help you answer your questions.