Concept

Subculture (biology)

Related publications (14)

Development of 3,5-Dinitrobenzylsulfanyl-1,3,4-oxadiazoles and Thiadiazoles as Selective Antitubercular Agents Active Against Replicating and Nonreplicating Mycobacterium tuberculosis

Stewart Cole, Anthony Vocat, Edina Rita Székely

Herein, we report the discovery and structure activity relationships of 5-substituted-2-[(3,5-dinitrobenzyl)-sulfanyl]-1,3,4-oxadiazoles and 1,3,4-thiadiazoles as a new class of antituberculosis agents. The majority of these compounds exhibited outstanding ...

American Chemical Society2016

Echovirus 11 mutants with increased resistance to common water disinfectants

Tamar Kohn, Anna Carratala Ripolles, Virginie Bachmann, Qingxia Zhong

Common water disinfectants like chlorine have been reported to select for resistant viruses. Yet, only little attention has been devoted to characterizing disinfection resistance. The goal of this study was to produce disinfectant-resistant viruses and to ...

2016

Echovirus 11 mutants with increased resistance to common water disinfectants

Tamar Kohn, Anna Carratala Ripolles, Virginie Bachmann, Qingxia Zhong

2016

Recombinant protein production from stable mammalian cell lines and pools

David Hacker, Sowmya Balasubramanian

We highlight recent developments for the production of recombinant proteins from suspension-adapted mammalian cell lines. We discuss the generation of stable cell lines using transposons and lentivirus vectors (non-targeted transgene integration) and site- ...

Elsevier2016

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The absorption of dietary calcium through the intestinal barrier is essential for maintaining health in general and especially of the bone system. We propose a microfluidic model that studies free calcium (Ca2+) transport through a confluent monolayer of C ...

Royal Soc Chemistry2014

Actin filament dynamics impacts keratinocyte stem cell maintenance

Yann Barrandon

Cultured human epidermal keratinocyte stem cells (holoclones) are crucial for regenerative medicine for burns and genetic disorders. In serial culture, holoclones progressively lose their proliferative capacity to become transient amplifying cells with lim ...

Wiley Open Access2013

PHOTOCATALYTIC DISINFECTION TREATMENTS: VIABILITY, CULTIVABILITY AND METABOLIC CHANGES OF E. coli USING DIFERENT MESUREMENTS METHODS

César Pulgarin, Julien Wist

In this work, the oxidative stress was produced by the illumination of either fixed TiO2 or suspended TiO2. and the relation between cultivability and viability under oxidative conditions was studied using a Direct Viable Count (DVC) and Fluorescence In Si ...

2011

The behaviour of cells cultured within three-dimensional (3D) structures rather than onto two-dimensional (2D) culture plastic more closely reflects their in vivo responses. Consequently, 3D culture systems are becoming crucial scientific tools in cancer c ...

Elsevier2010

Recombinant cell line generation by standard transfection techniques is a time-consuming and labor-intensive process often leading to an unpredictable outcome as transgene integration is a rare, random event. Consequently, the population of cells obtained ...

EPFL2010

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Repeated passaging in conventional cell culture reduces pluripotency and proliferation capacity of human mesenchymal stem cells (MSC). We introduce an innovative cell culture method whereby the culture surface is dynamically enlarged during cell proliferat ...

2008

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