Concept

Fluorescent tag

Related publications (325)

PhotoMEA: An opto-electronic biosensor for monitoring in vitro neuronal network activity

PhotoMEA is a biosensor useful for the analysis of an in vitro neuronal network, fully based on optical methods. Its function is based on the stimulation of neurons with caged glutamate and the recording of neuronal activity by Voltage-Sensitive fluorescen ...

Elsevier2007

FABRICATION OF NANOPORES IN SIN MEMBRANES USING FIB

Jürgen Brugger, Christian Santschi

Pressing adhering live cells onto the nanostructured SiN substrate and subsequent removal, planar cell membrane sheets (CMSs) were transferred in a well-defined orientation onto the SiN support. Using perforated thin SiN membranes both, extracellular and i ...

2006

The Colored Revolution of Bioimaging

Michaël Unser, Cédric René Jean Vonesch, François Aguet

With the recent development of fluorescent probes and new high-resolution microscopes, biological imaging has entered a new era and is presently having a profound impact on the way research is being conducted in the life sciences. Biologists have come to d ...

IEEE2006

Covalent labeling of cell-surface proteins for in-vivo FRET studies

Horst Vogel, Kai Johnsson, Rudolf Hovius, Jean-Manuel Segura, Nathalie George, Pedro Pascoal, Bruno Meyer

Fluorescence resonance energy transfer (FRET) is a powerful technique to reveal interactions between membrane proteins in live cells. Fluorescence labeling for FRET is typically performed by fusion with fluorescent proteins (FP) with the drawbacks of a lim ...

2006

Systemic Distribution and Elimination of Plain and with Cy3.5 Functionalized Poly(vinyl alcohol) Coated Superparamagnetic Maghemite Nanoparticles After Intraarticular Injection in Sheep In Vivo

Heinrich Hofmann, Alke Fink, Benedikt Steitz

PVA coated and fluorescent dye (Cy3.5) functionalized vinyl alcohol/vinyl amine copolymer coated superparamagnetic iron oxide nanoparticles (SPION) were evaluated for systemic distribution and elimination after intraarticular injection in sheep. Observatio ...

2006

Gene Expression in Synovial Membrane Cells After Intraarticular Delivery of Plasmid-Linked Superparamagnetic Iron Oxide Particles—A Preliminary Study in Sheep

Heinrich Hofmann, Alke Fink, Benedikt Steitz

This study evaluated in vivo gene deliver y and subsequent gene expression within cells of the synovium in the presence of static and pulsating magnetic field application following intraar ticular injection of super paramagnetic iron oxide nanopar ticles l ...

2006

Cell Membranes Suspended Across Nanoaperture Arrays

Jürgen Brugger, Horst Vogel, Christian Santschi, Christophe Danelon, Jean-Baptiste Perez

The authors present a method for spreading large (> 100 mm2) cell membrane fragments across nanoapertures in planar supports. Electron-beam and focused-ion-beam lithog. were used to fabricate arrays of 50-600 nm diam. holes in free-standing silicon nitride ...

2006

Glucose and lactate are equally effective in energizing activity-dependent synaptic vesicle turnover in purified cortical neurons

Pierre Magistretti, Jean-Yves Chatton

This study examines the role of glucose and lactate as energy substrates to sustain synaptic vesicle cycling. Synaptic vesicle turnover was assessed in a quantitative manner by fluorescence microscopy in primary cultures of mouse cortical neurons. An elect ...

Elsevier2006

Development and functionalisation of Sb doped SnO2 thin films for DNA biochip applications

Carlotta Guiducci, Olivier Renault

In view of developing DNA biochips based on electric detection, well controlled DNA functionalisation of conductive substrate electrode is a challenging process. In this study, the whole process of functionalisation onto home made conductive films with con ...

2006

Semiconductor oxide based electrodes for the label-free electrical detection of DNA hybridization: Comparison between Sb doped SnO2 and CdIn2O4

Carlotta Guiducci

First results are reported regarding the design, fabrication and operation of a DNA biochip based on a semiconductor oxide electrode that employs label-free electrical detection of the DNA hybridization. The same process of DNA functionalisation, including ...

2006

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