Concept

Chromatin immunoprecipitation

Related publications (61)

Development of chemical biology tools to reveal the function and organization of the silenced chromatin state

Effector proteins are recruited to chromatin via transient interactions between their reader domains and histone post-translational modification (PTM) patterns. These interactions form signaling pathways that control gene expression or repression and which ...

EPFL2020

KAP1 targets actively transcribed genomic loci to exert pleomorphic effects on RNA polymerase II activity

Didier Trono, Evaristo Jose Planet Letschert, Marco Cassano, Annamaria Kauzlaric, Suk Min Jang

KAP1 (KRAB-associated protein 1) is best known as a co-repressor responsible for inducing heterochromatin formation, notably at transposable elements. However, it has also been observed to bind the transcription start site of actively expressed genes. To a ...

2020

The role of OCT4 and SOX2 in regulating chromatin accessibility and cell fate across the cell cycle in embryonic stem cells

Torgil Elias Friman

Precise spatiotemporal regulation of gene expression is essential for development and homeostasis of complex organisms. This is achieved in large part by sequence-specific transcription factors (TF) that bind to genomic regulatory elements to activate or r ...

EPFL2019

The Ovulatory Signal Precipitates LRH-1 Transcriptional Switching Mediated by Differential Chromatin Accessibility

Kristina Schoonjans

In the ovary, follicular growth and maturation are complicated processes that involve a series of morphological and physiological changes in oocytes and somatic cells leading to ovulation and luteinization, essential processes for fertility. Given the comp ...

CELL PRESS2019

Mitotic chromosome binding predicts transcription factor properties in interphase

David Michael Suter, Cédric Deluz, Torgil Elias Friman, Andrea Brigitta Alber, Mahé Cécile Raccaud

Mammalian transcription factors (TFs) differ broadly in their nuclear mobility and sequence-specific/non-specific DNA binding. How these properties affect their ability to occupy specific genomic sites and modify the epigenetic landscape is unclear. The as ...

2019

Analytical Approaches for Monitoring DNA–Protein Interactions

Ardemis Anoush Boghossian, Shang-Jung Wu

DNA–protein interactions play a critical role in cellular regulation. We herein review existing analytical methods for investigating these interactions, highlighting methods such as chromatin immunoprecipitation and yeast-one-hybrid that are used to identi ...

2019

A Modular Ligation Strategy for Asymmetric Bivalent Nucleosomes Trimethylated at K36 and K27

Beat Fierz, Nora Guidotti, Andreas Linus Bachmann, Carolin Christine Lechner

In nature, individual histones in the same nucleosome can carry identical (symmetric) or different (asymmetric) post-translational modification (PTM) patterns, increasing the combinatorial complexity. Embryonic stem cells exhibit bivalent nucleosomes, some ...

WILEY-V C H VERLAG GMBH2019

Biophysics of Chromatin Dynamics

Beat Fierz

Nucleosomes and chromatin control eukaryotic genome accessibility and thereby regulate DNA processes, including transcription, replication, and repair. Conformational dynamics within the nucleosome and chromatin structure play a key role in this regulatory ...

ANNUAL REVIEWS2019

Crosslinking Allosteric Sites on the Nucleosome

Paul Joseph Dyson, Lucinda Kate Batchelor, Thibaud Edouard Alistair von Erlach

Targeting defined histone protein sites in chromatin is an emerging therapeutic approach that can potentially be enhanced by allosteric effects within the nucleosome. Here we characterized a novel hetero-bimetallic compound with a design based on a nucleos ...

WILEY-V C H VERLAG GMBH2019

Deciphering the chromatin binding mechanisms of human HP1 variants at the single-molecule level

Louise Catherine Bryan

In eukaryotic cells, DNA is tightly packed in the form chromatin. The basic structure of chromatin is a nucleosome composed of 147 bp DNA wrapped around eight histone proteins; two copies of H2A, H2B, H3 and H4. These histone proteins are decorated with pa ...

EPFL2018

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