Concept

Cloning vector

Related publications (130)

Helical-Track Bioreactors for Bacterial, Mammalian and Insect Cell Cultures

Florian Maria Wurm, David Hacker, Lucia Baldi Unser, Stéphanie Tissot, Clara Joséphine Douet

We investigated the cultivation of bacterial, mammalian and insect cells in an orbitally-shaken 250-mL disposable tube that incorporates a helical track (HT) on its inside wall. The mass transfer coefﬁcient of oxygen (kLa) was 200%–400% higher in the HT tu ...

2013

MAR-mediated integration of plasmid vectors for in vivo gene transfer and regulation

Andrzej Kulik, Nicolas Mermod, Stefania Puttini, Déborah Ley, Damien Saugy

Background: The in vivo transfer of naked plasmid DNA into organs such as muscles is commonly used to assess the expression of prophylactic or therapeutic genes in animal disease models. Results: In this study, we devised vectors allowing a tight regulatio ...

BioMed Central2013

MAR Elements and Transposons for Improved Transgene Integration and Expression

Nicolas Mermod, Déborah Ley, Niamh Harraghy

Reliable and long-term expression of transgenes remain significant challenges for gene therapy and biotechnology applications, especially when antibiotic selection procedures are not applicable. In this context, transposons represent attractive gene transf ...

2013

Switching Ripple Characteristics of Space Vector PWM Schemes for Five-Phase Two-Level Voltage Source Inverters - Part 2: Current Ripple

Drazen Dujic

Flux harmonic distortion factors (HDFs) of two space vector pulse width modulation (SVPWM) schemes, aimed at sinusoidal output voltage generation with five-phase voltage source inverters, have been evaluated in Part 1 of this paper. Analytical consideratio ...

2011

Developement of a gateway compatible system for speciﬁc DNA integration in mouse embryonic stem cells and evaluation of the effect of nucleosome positioning on gene expression

Julien Bryois

An emerging model views gene regulation as a competition for regulatory sequences between transcription factors and nucleosomes at thermodynamic equilibrium. A computational model of the DNA affinity of nucleosomes was recently developed allowing to predic ...

2010

A dual promoter lentiviral vector for the in vivo evaluation of gene therapeutic approaches to axon regeneration after spinal cord injury

The identification of axon growth-promoting genes, and overexpression of these genes in central nervous system (CNS) neurons projecting to the spinal cord, has emerged as one potential approach to enhancing CNS regeneration. Assessment of the regenerative ...

2010

Methane- and ammonia-oxidizing bacteria at the chemocline of Lake Kinneret (Israel)

The vertical distribution of methane- and ammonia-oxidizing bacteria (MOB and AOB, respectively), and the physicochemical conditions in the chemocline of Lake Kinneret (Israel) were studied at a resolution of 10 cm from 16.2 to 17.7 m depth. Profiles of th ...

2010

Structure-function analysis points to a polynucleotide-accommodating groove essential for APOBEC3A restriction activities

Didier Trono, Priscilla Turelli, Amalio Telenti, Shyam Sunder Peddi, Olivier Michielin

Members of the human APOBEC3 family of editing enzymes can inhibit various mobile genetic elements. APOBEC3A (A3A) can block the retrotransposon LINE-1 and the parvovirus adeno-associated virus type 2 (AAV-2) but does not inhibit retroviruses. In contrast, ...

American Society for Microbiology2010

Identification of genetic insulator elements to increase the safety of viral gene therapy vectors

Armelle Gaussin

Haematopoeitic system diseases, both acquired and inherited, can be currently cured by allogeneic haematopoietic stem cell transplantation. This treatment provides highly successful immune function recovery for patients receiving grafts of HLA-compatible d ...

EPFL2010

Current ripple in inverter-fed five-phase drives with space-vector PWM

Drazen Dujic

A number of PWM techniques have been developed in recent times for five-phase voltage source inverters (VSIs), which enable sinusoidal output voltage generation. Relatively little attention has been however paid to the current ripple caused by these PWM me ...

IEEE2010

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