Concept

Lysis

Related publications (36)

Portable automated osmolality and pH adjusting apparatus for pretreatment of environmental water samples delivered into a cell-based biosensor

Nico de Rooij, Peter Van der Wal, Sara Talaei

Using our portable in-line fluidic pretreatment apparatus we have adjusted the osmolality and pH of Swiss river samples automatically without any dilution or degradation of the samples. The samples will be tested by means of a cell-based water quality bios ...

2012

Mechanism of photocatalytic bacterial inactivation on TiO2 films involving cell-wall damage and lysis

César Pulgarin, Juan Kiwi

This article addresses the cell wall damage of Escherichia coil (from now on E. coil) by TiO2 suspensions. The dynamics of TiO2 photocatalysis by thin films layers is described. The films were characterized by FTIR spectroscopy and atomic force microscopy ...

Elsevier2012

Multiple-frequency impedance measurements in continuous flow for automated evaluation of yeast cell lysis

Philippe Renaud, Niccolo Piacentini, Willyan Hasenkamp Carreira

This paper reports on an electrical lysis device integrating multiple-frequency impedance measurements for automated evaluation of the lysis efficiency and counting of the lysed cells. Conventional techniques use chemical reagents to dissolve the cell memb ...

Elsevier2012

Single-Cell Analysis of Mycobacterial Persistence Using Microfabricated Tools

Meltem Elitas

Bacterial cells behave as individuals despite being genetically identical and subject to the same environment. Although the underlying mechanisms of cellular individuality are not well understood, temporal variation of gene expression and protein levels in ...

EPFL2012

Multiple-frequency Impedance Measurements in Continuous Flow for the Evaluation of Electrical Lysis of Yeast Cells

Philippe Renaud, Niccolo Piacentini, Willyan Hasenkamp Carreira

his paper reports on an electrical lysis device with integrated cell counting and evaluation of the lysis efficiency by multiplefrequency impedance measurements. Conventional techniques use chemical reagents to lyse cells, causing unwanted interference wit ...

Elsevier2010

Cell sorting by dielectrophoresis for evaluation of lysis efficiency in continuous flow

Philippe Renaud, Niccolo Piacentini

This paper reports on a device capable of electrical cell lysis and evaluation of lysis efficiency in continuous flow using dielectrophoretic cell sorting. The device uses an innovative design, called liquid electrodes, to perform both cell lysis and cell ...

2010

Efficient oxygen transfer by surface aeration in shaken cylindrical containers for mammalian cell cultivation at volumetric scales up to 1,000 liters

Alfio Quarteroni, Florian Maria Wurm, David Hacker, Xiuwei Zhang, Nicola Parolini, Mathieu Benjamin Stettler, Marco Perrone, Dario De Sanctis

Cylindrical containers agitated by orbital shaking are being developed as simple and cost-effective bioreactor systems for the cultivation of mammalian cells. Here the oxygen transfer capacities of containers with nominal volumes from 50 mL to 2000 L were ...

2009

Genome-wide regulon and crystal structure of BlaI (Rv1846c) from Mycobacterium tuberculosis

Stewart Cole, Claudia Sala

Comparative genomics with Staphylococcus aureus suggested the existence of a regulatory system governing beta-lactamase (BlaC) production in Mycobacterium tuberculosis. The crystal structure of Rv1846c, a winged helix regulator of previously unknown functi ...

2009

Selective cross-linking of interacting proteins using self-labeling tags

Kai Johnsson, Grazvydas Lukinavicius, Kui Thong Tan

We have designed molecules that permit the selective cross-linking (S-CROSS) of interacting proteins in cell lysates and the sensitive detection of the trapped complexes through in-gel fluorescence scanning. S-CROSS requires the expression of the putative ...

American Chemical Society2009

Mechanisms of pathogen entry through the endosomal compartments

Françoise Gisou van der Goot Grunberg

Several pathogens - bacteria, viruses and parasites - must enter mammalian cells for survival, replication and immune-system evasion. These pathogens generally make use of existing cellular pathways that are designed for nutrient uptake, receptor downregul ...

2006

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