Concept

Negative stain

Related publications (32)

Identification of a Dps contamination in Mitomycin-C–induced expression of Colicin Ia

Colicins are bacterial toxins targeting Gram-negative bacteria, including E. coli and related Enterobacteriaceae strains. Some colicins form ion-gated pores in the inner membrane of attacked bacteria that are lethal to their target. Colicin Ia was the firs ...

2021

Unraveling the complexity of amyloid polymorphism using gold nanoparticles and cryo-EM

Francesco Stellacci, Hilal Lashuel, Paulo Henrique Jacob Silva, Nadine Aït Bouziad, Anass Chiki, Zekiye Pelin Güven, Urszula Beata Cendrowska, Sophie Vieweg, Marie Mueller

Increasing evidence suggests that amyloid polymorphism gives rise to different strains of amyloids with distinct toxicities and pathology-spreading properties. Validating this hypothesis is challenging due to a lack of tools and methods that allow for the ...

NATL ACAD SCIENCES2020

HISTOBREAST, a collection of brightfield microscopy images of Haematoxylin and Eosin stained breast tissue

Tiberiu Totu

Modern histopathology workflows rely on the digitization of histology slides. The quality of the resulting digital representations, in the form of histology slide image mosaics, depends on various specific acquisition conditions and on the image processing ...

2020

Picture of the wet electron: a localized transient state in liquid water

Alfredo Pasquarello, Francesco Ambrosio, Michele Pizzochero

A transient state of the excess electron in liquid water preceding the development of the solvation shell, the so-called wet electron, has been invoked to explain spectroscopic observations, but its binding energy and atomic structure have remained highly ...

2019

Design and Synthesis of Gatekeeper Coated Dendritic Silica/Titania Mesoporous Nanoparticles with Sustained and Controlled Drug Release Properties for Targeted Synergetic Chemo-Sonodynamic Therapy

Saba Rezakhani, Samira Malekmohammadi

New dendritic silica/titania mesoporous nanoparticles (DSTNs) loaded with curcumin (CUR) were synthesized and coated with polyethylenimine-folic acid groups (PEI-FA) for an ultrasound (US)-triggered drug release and combined chemo-sonodynamic therapy. The ...

2019

Miniaturized Sample Preparation for Transmission Electron Microscopy

Henning Paul-Julius Stahlberg, Anastasia Syntychaki

Due to recent technological progress, cryo-electron microscopy (cryo-EM) is rapidly becoming a standard method for the structural analysis of protein complexes to atomic resolution. However, protein isolation techniques and sample preparation methods for E ...

MyJove Corporation2018

A Cell Atlas for the Mouse Brain

Henry Markram, Daniel Keller, Marc-Oliver Gewaltig

Despite vast numbers of studies of stained cells in the mouse brain, no current brain atlas provides region-by-region neuron counts. In fact, neuron numbers are only available for about 4% of brain of regions and estimates often vary by as much as 3-fold. ...

2018

Compact lensless digital holography

Manon Rostykus

Microscopy is of high interest for biology since it allows imaging features that are too small to be seen with naked eyes. However, cells are mostly transparent to visible and infrared light which makes it difficult to see with a traditional microscope. To ...

EPFL2018

Controlled Charging of Ferroelastic Domain Walls in Oxide Ferroelectrics

Tomas Sluka, Li Jin, Ludwig Feigl, Xiankui Wei, Barbara Maraston Fraygola

Conductive domain walls (DWs) in ferroic oxides as device elements are a highly attractive research topic because of their robust and agile response to electric field. Charged DWs possessing metallic-type conductivity hold the highest promises in this aspe ...

Amer Chemical Soc2017

Amyloid Fibril Polymorphism: Almost Identical on the Atomic Level, Mesoscopically Very Different

Henning Paul-Julius Stahlberg

Amyloid polymorphism of twisted and straight beta-endorphin fibrils was studied by negative-stain transmission electron microscopy, scanning transmission electron microscopy, and solid-state nuclear magnetic resonance spectroscopy. Whereas fibrils assemble ...

American Chemical Society (ACS)2017

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