Concept

Immunofluorescence

Related publications (168)

Microtextured Substrates and Microparticles Used as in Situ Lenses for On-Chip Immunofluorescence Amplification

We propose a concept for enhancing the fluorescent detection signal of on-chip immunoassays by using three-dimensional 3 and 8 mu m size microtextured structures as substrates for the assay, which allows exploitation of a large number of fluorophores withi ...

Amer Chemical Soc2013

Substrates for Improved Live-Cell Fluorescence Labeling of SNAP-tag

Kai Johnsson, Luc Reymond, Grazvydas Lukinavicius

The SNAP-tag labeling technology provides a simple, robust, and versatile approach to the imaging of fusion proteins for a wide range of experimental applications. Owing to the specific and covalent nature of the labeling reaction, SNAP-tag is well suited ...

Bentham Science Publ Ltd2013

Functional and Structural Imaging with Optical Coherence Microscopy

Arno Pino Bouwens

Fluorescence microscopy techniques are well established research tools and have proven their use in a large variety of biomedical applications. Microscopic molecular contrast is achieved by imaging fluorescent dyes that bind specifically to a molecule of i ...

EPFL2013

3-D PSF fitting for fluorescence microscopy: implementation and localization application

Michaël Unser, Daniel Sage, François Aguet, Hagay Kirshner

Localization microscopy relies on computationally efficient Gaussian approximations of the point spread function for the calculation of fluorophore positions. Theoretical predictions show that under specific experimental conditions, localization accuracy i ...

Wiley-Blackwell2013

Structural and Functional Stochastic Super-Resolution Microscopy

Stefan Geissbühler

Super-resolution fluorescence microscopy is a promising tool with the potential to strengthen our understanding of living processes. Based on the ability to switch fluorophores on and off in an either deterministic or stochastic manner, the fundamental lim ...

EPFL2013

SPAD array camera for localization based super resolution microscopy

Edoardo Charbon, Claudio Bruschini, Francesco Regazzoni, Samuel Burri

Super resolution microscopy by localization is a stochastic based approach, where the resolution is determined by the localization accuracy [1] [2] [3]. The accuracy of localization heavily depends on the statistics of the data obtained with a camera durin ...

2013

Self-assembled melamine microlens arrays for immunofluorescence enhancement

Martinus Gijs, Abdeljalil Sayah, Hüseyin Cumhur Tekin, Hui Yang

Self-assembled dielectric microlenses are used for focusing of the fluorescent signal emitted from a surface-based immunoassay, performed on silane micropatterns as assay substrates, to enhance the detection signal. In our model system, a fluorescent immun ...

2013

Use of red autofluorescence for monitoring prodiginine biosynthesis

Paul Guichard

Prodigiosin-like pigments or prodiginines (PdGs) are promising drugs owing to their reported antitumor, antibiotic, and immunosuppressive activities. These natural compounds are produced by several bacteria, including Streptomyces coelicolor and Serratia m ...

Elsevier Science Bv2013

Benchmarking Image-Processing Algorithms for Biomicroscopy: Reference Datasets and Perspectives

Michaël Unser, Daniel Sage, Cédric René Jean Vonesch, Hagay Kirshner, Stamatios Lefkimmiatis

As the field of bioimage informatics matures, the issue of the validation of image reconstruction algorithms and the definition of proper performance criteria becomes more pressing. In this work, we discuss benchmarking aspects of fluorescence microscopy q ...

EUSIPCO2013

Resolution Doubling in 3D-STORM Imaging through Improved Buffers

Pierre Gönczy, Suliana Manley, Debora Keller

Super-resolution imaging methods have revolutionized fluorescence microscopy by revealing the nanoscale organization of labeled proteins. In particular, single-molecule methods such as Stochastic Optical Reconstruction Microscopy (STORM) provide resolution ...

Public Library of Science2013

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