Publications related to Site-directed mutagenesis

Alternative dimerization is required for activity and inhibition of the HEPN ribonuclease RnlA

The rnlAB toxin-antitoxin operon from Escherichia co/ifunctions as an anti-phage defense system. RnlA was identified as a member of the HEPN (Higher Eukaryotes and Prokaryotes Nucleotide-binding domain) superfamily of ribonucleases. The activity of the tox ...

OXFORD UNIV PRESS2021

Generation of a Large Peptide Phage Display Library by Self-Ligation of Whole-Plasmid PCR Product

Christian Heinis, Xudong Kong, Vanessa Carle, Cristina Diaz Perlas, Kaycie Marie Butler

The success of phage display, used for developing target-specific binders based on peptides and proteins, depends on the size and diversity of the library screened, but generating large libraries of phage-encoded polypeptides remains challenging. New pepti ...

2020

Site-Specific Protein Conjugation onto Fluorescent Single-Walled Carbon Nanotubes

Ardemis Anoush Boghossian, Shang-Jung Wu, Vitalijs Zubkovs

Semiconducting single-walled carbon nanotubes (SWCNTs) are among the few photostable optical emitters that are ideal for sensing, imaging, drug delivery, and monitoring of protein activity. These applications often require strategies for immobilizing prote ...

2020

Fryns type mesomelic dysplasia of the upper limbs caused by inverted duplications of the HOXD gene cluster

Denis Duboule

The HoxD cluster is critical for vertebrate limb development. Enhancers located in both the telomeric and centromeric gene deserts flanking the cluster regulate the transcription of HoxD genes. In rare patients, duplications, balanced translocations or inv ...

NATURE PUBLISHING GROUP2020

Single-molecule sensing of peptides and nucleic acids by engineered aerolysin nanopores

Aleksandra Radenovic, Matteo Dal Peraro, Chan Cao, Nuria Cirauqui Diaz, Alice Duperrex, Elena Buglakova

Nanopore sensing is a powerful single-molecule approach for the detection of biomolecules. Recent studies have demonstrated that aerolysin is a promising candidate to improve the accuracy of DNA sequencing and to develop novel single-molecule proteomic str ...

2019

Phosphorylation of the VAR2CSA extracellular region is associated with enhanced adhesive properties to the placental receptor CSA

Romain Hamelin, Florence Armand, Marc Moniatte

Plasmodium falciparum is the main cause of disease and death from malaria. P. falciparum virulence resides in the ability of infected erythrocytes (IEs) to sequester in various tissues through the interaction between members of the polymorphic P. falciparu ...

PUBLIC LIBRARY SCIENCE2019

alpha-Synuclein O-GlcNAcylation alters aggregation and toxicity, revealing certain residues as potential inhibitors of Parkinson's disease

Hilal Lashuel, Anne-Laure Mahul Mellier

A compelling link is emerging between the posttranslational modification O-GlcNAc and protein aggregation. A prime example is alpha-synuclein, which forms toxic aggregates that are associated with neurodegeneration in Parkinson's and related diseases. alph ...

NATL ACAD SCIENCES2019

Palmitoylation mediates membrane association of hepatitis E virus ORF3 protein and is required for infectious particle secretion

Françoise Gisou van der Goot Grunberg, Laurence Gouzi Abrami, Johann Mauron, Joël Oppliger, Maxime Matter, Noémie Oechslin

Hepatitis E virus (HEV) is a positive-strand RNA virus encoding 3 open reading frames (ORF). HEV ORF3 protein is a small, hitherto poorly characterized protein involved in viral particle secretion and possibly other functions. Here, we show that HEV ORF3 p ...

PUBLIC LIBRARY SCIENCE2018

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Site-directed mutagenesis

Alternative dimerization is required for activity and inhibition of the HEPN ribonuclease RnlA

Generation of a Large Peptide Phage Display Library by Self-Ligation of Whole-Plasmid PCR Product

Site-Specific Protein Conjugation onto Fluorescent Single-Walled Carbon Nanotubes

Fryns type mesomelic dysplasia of the upper limbs caused by inverted duplications of the HOXD gene cluster

Single-molecule sensing of peptides and nucleic acids by engineered aerolysin nanopores

Phosphorylation of the VAR2CSA extracellular region is associated with enhanced adhesive properties to the placental receptor CSA

A new approach to Cas9-based genome editing in Aspergillus niger that is precise, efficient and selectable

alpha-Synuclein O-GlcNAcylation alters aggregation and toxicity, revealing certain residues as potential inhibitors of Parkinson's disease

Design and validation of bioorthogonal probes for cellular disease models

Palmitoylation mediates membrane association of hepatitis E virus ORF3 protein and is required for infectious particle secretion

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Chat with Graph Search

Generation of a Large Peptide Phage Display Library by Self-Ligation of Whole-Plasmid PCR Product

Fryns type mesomelic dysplasia of the upper limbs caused by inverted duplications of the HOXD gene cluster

A new approach to Cas9-based genome editing in Aspergillus niger that is precise, efficient and selectable

Single-molecule sensing of peptides and nucleic acids by engineered aerolysin nanopores

Phosphorylation of the VAR2CSA extracellular region is associated with enhanced adhesive properties to the placental receptor CSA

Site-Specific Protein Conjugation onto Fluorescent Single-Walled Carbon Nanotubes

Design and validation of bioorthogonal probes for cellular disease models

Alternative dimerization is required for activity and inhibition of the HEPN ribonuclease RnlA

Palmitoylation mediates membrane association of hepatitis E virus ORF3 protein and is required for infectious particle secretion

alpha-Synuclein O-GlcNAcylation alters aggregation and toxicity, revealing certain residues as potential inhibitors of Parkinson's disease