Concept
Galactose
Related publications (36)
Identification of Mobility-Resolved N-Glycan Isomers
Thomas Rizzo, Robert Paul Pellegrinelli, Ahmed Ben Faleh, Stephan Warnke, Priyanka Bansal
ABSTRACT: Glycan analysis has evolved considerably during the last decade. The advent of high-resolution ion-mobility spectrometry has enabled the separation of isomers with only the slightest of structural differences. However, the ability to separate suc ...
AMER CHEMICAL SOC2022A New Strategy Coupling Ion-Mobility-Selective CID and Cryogenic IR Spectroscopy to Identify Glycan Anomers
Thomas Rizzo, Robert Paul Pellegrinelli, Ahmed Ben Faleh, Stephan Warnke, Eduardo Carrascosa Casado, Priyanka Bansal, Lei Yue
Determining the primary structure of glycans remains challenging due to their isomeric complexity. While high-resolution ion mobility spectrometry (IMS) has recently allowed distinguishing between many glycan isomers, the arrival-time distributions (ATDs) ...
2022Combining multi-stage ion mobility spectrometry with cryogenic infrared spectroscopy for glycan analysis
Structural analysis of biomolecules is essential to understanding biological processes. Among the various classes of biomolecules, glycans have gained significant attention recently due to their ubiquitous roles in biological systems. However, study of gly ...
EPFL2022Combining cryognic ion spectroscopy with ion mobility for the study of glycan fragmentation
The importance of glycans in biological processes are matched by their structural complexity. Coating the surfaceof most living cells, glycans play key roles in many biological processes, and the role they play is closely relatedto their structures. Struct ...
EPFL2021Identifying Mixtures of Isomeric Human Milk Oligosaccharides by the Decomposition of IR Spectral Fingerprints
Thomas Rizzo, Ahmed Ben Faleh, Stephan Warnke, Ali H Abikhodr, Vasyl Yatsyna
The analysis of glycans presents a significant challenge that arises from their isomeric heterogeneity. While high-resolution ion mobility spectrometry (IMS) has shown the ability to resolve subtly different glycan isomers, their unambiguous assignment rem ...
2021Equilibrium and non-equilibrium furanose selection in the ribose isomerisation network
Paolo De Los Rios, Daniel Maria Busiello, Shiling Liang, Francesco Piazza
The exclusive presence of beta -D-ribofuranose in nucleic acids is still a conundrum in prebiotic chemistry, given that pyranose species are substantially more stable at equilibrium. However, a precise characterisation of the relative furanose/pyranose fra ...
NATURE RESEARCH2021Unravelling the structures of sodiated beta-cyclodextrin and its fragments
Thomas Rizzo, Robert Paul Pellegrinelli, Eduardo Carrascosa Casado, Ali H Abikhodr
We present cryogenic infrared spectra of sodiated beta-cyclodextrin beta-CD + Na, a common cyclic oligosaccharide, and its main dissociation products upon collision-induced dissociation (CID). We characterize the parent ions using high-resolution ion ...
ROYAL SOC CHEMISTRY2021Cryogenic Ion Spectroscopy for Identification of Monosaccharide Anomers
Thomas Rizzo, Valeriu Scutelnic
We combine conformer-selective, cryogenic infrared spectroscopy, quantum mechanical computations, and 18O substitution at the reducing end to determine the structural preferences of protonated glucosamine in the gas phase. Cryogenic infrared-infrared (IR-I ...
2019Cryogenic Vibrational Spectroscopy Provides Unique Fingerprints for Glycan Identification
Thomas Rizzo, Michael Zachary Kamrath, Chiara Masellis, Neelam Khanal
The structural characterization of glycans by mass spectrometry is particularly challenging. This is because of the high degree of isomerism in which glycans of the same mass can differ in their stereochemistry, attachment points, and degree of branching. ...
2017Toward Quantitative Measurements of Enzyme Kinetics by Dissolution Dynamic Nuclear Polarization
Geoffrey Bodenhausen, Aurélien Bornet, Sami Jannin, Jonas Milani, Daniel Abergel
Dissolution dynamic nuclear polarization (D-DNP) experiments enabled us to study the kinetics of the enzymatic phosphorylation reaction of glucose to form glucose-6-phosphate (G6P) by hexokinase (HK), with or without the presence of an excess of G6P, which ...
2014