Publications related to Transformation (genetics)

Regulatory elements involved in the expression of competence genes in naturally transformable Vibrio cholerae

BackgroundThe human pathogen Vibrio cholerae normally enters the developmental program of natural competence for transformation after colonizing chitinous surfaces. Natural competence is regulated by at least three pathways in this organism: chitin sensing ...

BioMed Central2014

Extending the Schizosaccharomyces pombe Molecular Genetic Toolbox

Viesturs Simanis, Andrea Krapp

Targeted alteration of the genome lies at the heart of the exploitation of S. pombe as a model system. The rate of analysis is often determined by the efficiency with which a target locus can be manipulated. For most loci this is not a problem, however for ...

Public Library of Science2014

A transcriptional regulator linking quorum sensing and chitin induction to render Vibrio cholerae naturally transformable

Melanie Blokesch, Mirella Lo Scrudato

The human pathogen Vibrio cholerae is an aquatic bacterium associated with zooplankton and their chitinous exoskeletons. On chitinous surfaces, V. cholerae initiates a developmental programme, known as natural competence, to mediate transformation, which i ...

Oxford University Press2013

Cues and regulatory pathways involved in natural competence and transformation in pathogenic and environmental Gram-negative bacteria

Melanie Blokesch, Patrick Martin Seitz

Bacterial genomics is flourishing, as whole-genome sequencing has become affordable, readily available, and rapid. As a result, it has become clear how frequently horizontal gene transfer (HGT) occurs in bacteria. The potential implications are highly sign ...

Wiley-Blackwell2013

DNA-uptake machinery of naturally competent Vibrio cholerae

Melanie Blokesch, Patrick Martin Seitz

Natural competence for transformation is a mode of horizontal gene transfer that is commonly used by bacteria to take up DNA from their environment. As part of this developmental program, so-called competence genes, which encode the components of a DNA-upt ...

National Academy of Sciences2013

Overexpression of the tcp Gene Cluster Using the T7 RNA Polymerase/Promoter System and Natural Transformation-Mediated Genetic Engineering of Vibrio cholerae

Melanie Blokesch

The human pathogen and aquatic bacterium Vibrio cholerae belongs to the group of naturally competent bacteria. This developmental program allows the bacterium to take up free DNA from its surrounding followed by a homologous recombination event, which allo ...

Public Library of Science2013

Cell Transformations and Physical Design Techniques for 3D Monolithic Integrated Circuits

Giovanni De Micheli, Shashi Kanth Bobba

3D monolithic integration (3DMI), also termed as sequential integration, is a potential technology for future gigascale circuits. In 3DMI technology the 3D contacts, connecting different active layers, are in the order of few 100 nm. Given the advantage of ...

2013

MAR-mediated integration of plasmid vectors for in vivo gene transfer and regulation

Andrzej Kulik, Nicolas Mermod, Stefania Puttini, Déborah Ley, Damien Saugy

Background: The in vivo transfer of naked plasmid DNA into organs such as muscles is commonly used to assess the expression of prophylactic or therapeutic genes in animal disease models. Results: In this study, we devised vectors allowing a tight regulatio ...

BioMed Central2013

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Transformation (genetics)

Regulatory elements involved in the expression of competence genes in naturally transformable Vibrio cholerae

Extending the Schizosaccharomyces pombe Molecular Genetic Toolbox

A transcriptional regulator linking quorum sensing and chitin induction to render Vibrio cholerae naturally transformable

Cues and regulatory pathways involved in natural competence and transformation in pathogenic and environmental Gram-negative bacteria

DNA-uptake machinery of naturally competent Vibrio cholerae

Overexpression of the tcp Gene Cluster Using the T7 RNA Polymerase/Promoter System and Natural Transformation-Mediated Genetic Engineering of Vibrio cholerae

Metabolomics of hexachlorocyclohexane (HCH) transformation: ratio of LinA to LinB determines metabolic fate of HCH isomers

Randomized codon mutagenesis reveals that the HIV Rev arginine-rich motif is robust to substitutions and that double substitution of two critical residues alters specificity

Cell Transformations and Physical Design Techniques for 3D Monolithic Integrated Circuits

MAR-mediated integration of plasmid vectors for in vivo gene transfer and regulation

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Chat with Graph Search

Metabolomics of hexachlorocyclohexane (HCH) transformation: ratio of LinA to LinB determines metabolic fate of HCH isomers

Cues and regulatory pathways involved in natural competence and transformation in pathogenic and environmental Gram-negative bacteria

Regulatory elements involved in the expression of competence genes in naturally transformable Vibrio cholerae

Extending the Schizosaccharomyces pombe Molecular Genetic Toolbox

MAR-mediated integration of plasmid vectors for in vivo gene transfer and regulation

DNA-uptake machinery of naturally competent Vibrio cholerae

Randomized codon mutagenesis reveals that the HIV Rev arginine-rich motif is robust to substitutions and that double substitution of two critical residues alters specificity

Cell Transformations and Physical Design Techniques for 3D Monolithic Integrated Circuits

A transcriptional regulator linking quorum sensing and chitin induction to render Vibrio cholerae naturally transformable

Overexpression of the tcp Gene Cluster Using the T7 RNA Polymerase/Promoter System and Natural Transformation-Mediated Genetic Engineering of Vibrio cholerae