Concept

Transformation (genetics)

Related publications (124)

Systematic Analysis of Yeast Gene Expression by Synthetic Promoter Libraries

The precise tuning of gene expression levels is essential for optimal performance of transcriptional regulatory networks. Many regulatory components control promoter output, but transcription factor (TF) binding sites are amongst the most prominent. Beside ...

EPFL2013

Evidence for Two Different Regulatory Mechanisms Linking Replication and Segregation of Vibrio cholerae Chromosome II

Melanie Blokesch

Understanding the mechanisms that coordinate replication initiation with subsequent segregation of chromosomes is an important biological problem. Here we report two replication-control mechanisms mediated by a chromosome segregation protein, ParB2, encode ...

Public Library of Science2013

TransFLP - A Method to Genetically Modify Vibrio cholerae Based on Natural Transformation and FLP-recombination

Melanie Blokesch

Several methods are available to manipulate bacterial chromosomes(1-3). Most of these protocols rely on the insertion of conditionally replicative plasmids (e.g. harboring pir-dependent or temperature-sensitive replicons(1,2)). These plasmids are integrate ...

2012

The Regulatory Network of Natural Competence and Transformation of Vibrio cholerae

Melanie Blokesch, Mirella Lo Scrudato

The human pathogen Vibrio cholerae is an aquatic bacterium frequently encountered in rivers, lakes, estuaries, and coastal regions. Within these environmental reservoirs, the bacterium is often found associated with zooplankton and more specifically with t ...

2012

A quorum sensing-mediated switch contributes to natural transformation of Vibrio cholerae

Melanie Blokesch

There is a fundamental gap in our understanding of how horizontal gene transfer contributes to the enormous range of genetic variations that are observed among bacteria. The objective of our study was to better understand how the acquisition of genetic mat ...

2012

Chitin colonization, chitin degradation and chitin-induced natural competence of Vibrio cholerae are subject to catabolite repression

Melanie Blokesch

Although Vibrio cholerae is a human pathogen its primary habitat are aquatic environments. In this environment, V. cholerae takes advantage of the abundance of zooplankton, whose chitinous exoskeletons provide a nutritious surface. Chitin also induces the ...

Wiley-Blackwell2012

Very High Throughput Electrical Cell Lysis and Extraction of Intracellular Compounds Using 3D Carbon Electrodes in Lab-on-a-Chip Devices

Philippe Renaud, Freddy Radtke, Rajwinder Lehal, Rodrigo Martinez Duarte

Here we present an electrical lysis throughput of 600 microliters per minute at high cell density (108 yeast cells per ml) with 90% efficiency, thus improving the current common throughput of one microliter per minute. We also demonstrate the extraction of ...

2012

Glycan variability on a recombinant IgG antibody transiently produced in HEK-293E cells

Florian Maria Wurm, Yury Tsybin, Lucia Baldi Unser, Sophie Nallet, Luca Fornelli, Simone Schmitt, Julien Parra

In this study, a recombinant monoclonal IgG antibody was produced by transient gene expression (TGE) in suspension-adapted HEK-293E cells. The objective of the study was to determine the variation in recombinant IgG yield and glycosylation in ten independe ...

Elsevier2012

Quorum Sensing Contributes to Natural Transformation of Vibrio cholerae in a Species-Specific Manner

Melanie Blokesch, Patrick Martin Seitz, Gaia Francesca Marianne Suckow

Although it is a human pathogen, Vibrio cholerae is a regular member of aquatic habitats, such as coastal regions and estuaries. Within these environments, V. cholerae often takes advantage of the abundance of zooplankton and their chitinous molts as a nut ...

American Society for Microbiology2011

Notch in T-ALL: new players in a complex disease

Freddy Radtke, Ute Koch

T cell acute lymphoblastic leukemia (T-ALL) is an aggressive hematopoietic malignancy of thymocytes affecting preferentially children and adolescents. The disease is heterogeneous and characterized by a large set of chromosomal and genetic alterations that ...

Elsevier2011

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