Concept

Gel electrophoresis of proteins

Related publications (40)

Capillary Electrophoresis as a Second Dimension to Isoelectric Focusing for Peptide Separation

Capillary zone electrophoresis and carrier ampholytes based capillary electrophoresis have been used as a second separation step to Off-Gel isoelectric focusing for the analysis of complex peptide mixtures. A tryptic digest of four proteins (bovine serum a ...

2007

The role of cisplatin and NAMI-A plasma-protein interactions in relation to combination therapy

Paul Joseph Dyson, Gianni Sava

The aim of the study is to evaluate the differences of protein binding of NAMI-A, a new ruthenium drug endowed with selective antimetastatic properties, and of cisplatin and to ascertain the possibility to use two drugs based on heavy metals in combination ...

2006

Electrohydrodynamic Properties of Succinoglycan as Probed by Fluorescence Correlation Spectroscopy, Protolytic Titration and Capillary Electrophoresis

The electrostatic, hydrodynamic and conformational properties of aqueous solutions of succinoglycan have been analyzed by fluorescence correlation spectroscopy (FCS), proton titration, and capillary electrophoresis (CE) over a large range of pH values and ...

2006

Micro ion analysis

Xiaoxia Bai

Due to the growing interest on micro total analytical system (μTAS), fast analysis of small amount samples with high throughout is highly desired. Conventional separation techniques, such as Liquid Chromatography, show a great potential for miniaturisation ...

EPFL2004

Peptide and protein characterization by high-rate electron capture dissociation Fourier transform ion cyclotron resonance mass spectrometry

Yury Tsybin

A review. The anal. utility of the electron capture dissocn. (ECD) technique, developed by McLafferty and co-workers, has substantially improved peptide and protein characterization using Fourier transform ion cyclotron resonance mass spectrometry (FTICR-M ...

2004

Analysis of Lipoproteins by Capillary Zone Electrophoresis in Microfluidic Devices: Assey Development and Surfaces Roughness Measurements

Nico de Rooij

The development of a new assay for lipoproteins by capillary electrophoresis in fused-silica capillaries and in glass microdevices is described in this paper. The separation of low-density (LDL) and high-density (HDL) lipoproteins by capillary zone electro ...

2002

Capillary electrophoresis and electron capture dissociation Fourier transform ion cyclotron resonance mass spectrometry for peptide mixture and protein digest analysis

Yury Tsybin

Recent advances in peptide fragmentation techniques and mass spectrometry have opened up the possibility of combining peptide sepn. techniques, such as capillary electrophoresis (CE) and capillary liq. chromatog., with Fourier transform ion cyclotron reson ...

2002

The generation of monoclonal antibodies against human peroxisome proliferator-activated receptors (PPARs)

Johan Auwerx

Monoclonal antibodies (Mabs) are valuable reagents for the purification, characterization and immunolocalization of proteins.In this study, we raised Mabs against human peroxisome proliferator-activated receptors (PPARs) using baculovirus particles display ...

2002

Polymer microfluidic chips for electrochemical and biochemical analyses

Joël Stéphane Rossier

Our recent developments concerning the fabrication of polymer microchips and their applications for biochemical analyses are reviewed. We first describe two methods of fabrication of polymer microfluidic chips, namely UV-laser photoablation and plasma etch ...

2002

Sample preconcentration by field amplification stacking for microchip-based capillary electrophoresis

Nico de Rooij

A microchip structure for field amplification stacking (FAS) was developed, which allowed the formation of comparatively long, volumetrically defined sample plugs with a minimal electrophoretic bias. Up to 20-fold signal gains were achieved by injection an ...

2001

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