Concept

Gel electrophoresis of proteins

Related publications (40)

Utilisation of the sol-gel technique for the development of novel stationary phases for capillary electrochromatography on a chip

Martinus Gijs, Abdeljalil Sayah, Dominique Solignac

Capillary electrochromatography (CEC) appears ideally suited for high performance separations at small scale, i.e. on a chip. Problems with the reproducible production of the required HPLC column, but also the lack of commercially available CEC instruments ...

2001

Analysis of glycosyl phosphatidylinositol-anchored proteins by two-dimensional gel electrophoresis

Françoise Gisou van der Goot Grunberg

The aim of this study was to characterize mammalian glycosyl phosphatidylinositol (GPI)-anchored proteins y two-dimensional gel electrophoresis using immobilized pH gradients. Analysis was performed on detergent-resistant membrane fractions of baby hamster ...

2000

Holographic refractive index detector for application in microchip-based separation systems

Nico de Rooij

A novel detection scheme for capillary electrophoresis on planar glass microchips is presented. The application of a holographic-based refractive index detector to the electrophoretic separation of carbohydrates is described. The microchip device consists ...

1998

High mobility group chromosomal protein 1 binds to the adeno-associated virus replication protein (Rep) and promotes Rep-mediated site-specific cleavage of DNA, ATPase activity and transcriptional repression

Peter Martin Beard

High mobility group protein 1 (HMG1) is an abundant non-histone chromosomal protein which plays a role in several nuclear events involving DNA. Here we demonstrate that HMG1 physically interacts with the human adeno-associated virus (AAV) Rep protein. HMG1 ...

1997

Phosphorylation of Serine Residues 3, 6, 10, and 13 Distinguishes Membrane Anchored from Soluble Glutamic Acid Decarboxylase 65 and Is Restricted to Glutamic Acid Decarboxylase 65

Steinunn Baekkeskov Hanahan

GAD65, the smaller isoform of the γ-aminobutyric acid-synthesizing enzyme glutamic acid decarboxylase is detected as an α/β doublet of distinct mobility on SDS-polyacrylamide gel electrophoresis. Glutamic acid decarboxylase (GAD) 65 is reversibly anchored ...

American Society for Biochemistry and Molecular Biology1997

Characterization of monoclonal IgA production and activity in hollow-fiber and fluidized-bed reactors

Urs von Stockar, Ian William Marison

An IgA-producing hybridoma cell line has been grown in two hollow-fiber reactors and one fluidized-bed reactor, using a serum-contg. medium and a protein-free medium. Comparisons have been made on the basis of product quantity (total IgA) and quality (acti ...

Elsevier1996

Systematic improvement of a chemically-defined protein-free medium for hybridoma growth and monoclonal antibody production

Urs von Stockar, Ian William Marison

A chemically-defined protein-free medium (FMX-Turbodoma) has been improved for the production of monoclonal IgA antibodies by hybridoma cells, using a systematic method. Cell growth rate, IgA production, activity and molecular weight pattern have been used ...

1996

SV40 activates transcription from the transferrin receptor promoter by inducing a factor which binds to the CRE/AP-1 recognition sequence

Peter Martin Beard, Nicole Paduwat

During the course of lytic infection by simian virus 40 (SV40), expression of both the viral late genes and certain host cellular genes is induced. The promoter of the cellular transferrin receptor (TR) gene contains a DNA sequence which is similar to the ...

1991

Regulation of transcription in vitro from herpes simplex virus genes

Peter Martin Beard

In vitro transcription assays were carried out by using as templates DNAs cut from the herpes simplex virus early glycoprotein D gene, the late glycoprotein C gene, the late VP5 gene, and the immediate-early ICP22 gene. Nuclear extracts from suspension cul ...

1986

Specific pancreatic beta-cell surface antigens recognized by a xenogenic antiserum

Steinunn Baekkeskov Hanahan

An antiserum (R4) from a rabbit immunized with suspensions of C57BL/61 ob/ob mouse islet cells contains antibodies which in a 125I-protein A radioligand assay can be demonstrated to bind to single cell suspensions of normal Naval Medical Research Institute ...

1982

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