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Conformational changes occurring upon membrane binding and subsequent insertion of staphylococcal alpha-toxin were studied using complementary spectroscopic techniques. Experimental conditions were established where binding could be uncoupled from membrane ...
Amino acid transport across cellular membranes is mediated by multiple transporters with overlapping specificities. We recently have identified the vertebrate proteins which mediate Na+-independent exchange of large neutral amino acids corresponding to tra ...
Colicins are unusual bacterial toxins because they are directed against close relatives of the producing strain. They kill their targets in one of three distinct ways; via a ribonuclease or deoxyribonuclease activity or by forming pores in the target cell' ...
Aquaporins form a large family of membrane channels involved in osmoregulation, Electron crystallography has shown monomers to consist of six membrane spanning a-helices confirming sequence based predictions. Surface exposed loops are the least conserved r ...
The smaller isoform of the GABA synthesizing enzyme glutamic acid decarboxylase, GAD65, is synthesized as a soluble protein that undergoes posttranslational modification(s) in the NH2-terminal region to become anchored to the membrane of small synaptic-lik ...
Aerolysin secreted by the human pathogen Aeromonas hydrophila belongs to a group of bacterial toxins that are hemolytic and form channels in biological membranes. The toxin is secreted as an inactive precursor proaerolysin that must be proteolytically proc ...
This paper describes the design, fabrication and experimental results of a new, low cost, high-performance silicon micropump developed for a disposable drug delivery system. The pump chip demonstrates linear and accurate (+/-5%) pumping characteristics for ...
A one-step procedure for the preparation of ion-selective membranes is described. The method employs the thermally induced gel crystallization of ultrahigh molecular weight poly(ethylene) (UHMW-PE) from a dilute xylene solution. After evaporation of the xy ...
The synthesis of a benzo-15-crown-5 functionalized α-olefin (4′-(undec-10-enylcarboxylate)benzo-15-crown-5, 5) and its terpolymerization with carbon monoxide and various α-olefins are described. A decline of the glass transition temperatures (Tg) of the co ...
Membrane proteins fulfill many central functions in the biological membrane. The insertion process of these proteins and their structure, which are intimately linked to their function, are not yet well understood. As a model we studied three proteins recon ...