Concept
Exocytosis
Related publications (73)
Cholesterol-Containing Liposomes Decorated With Au Nanoparticles as Minimal Tunable Fusion Machinery
Francesco Stellacci, Paulo Henrique Jacob Silva, Ester Canepa, Giulia Rossi
Membrane fusion is essential for the basal functionality of eukaryotic cells. In physiological conditions, fusion events are regulated by a wide range of specialized proteins, operating with finely tuned local lipid composition and ionic environment. Fusog ...
WILEY-V C H VERLAG GMBH2023Theoretical analysis of divalent cation effects on aptamer recognition of neurotransmitter targets
Nako Nakatsuka, Dmitry Momotenko, Annina Stuber
Aptamer-based sensing of small molecules such as dopamine and serotonin in the brain, requires characterization of the specific aptamer sequences in solutions mimicking the in vivo environment with physiological ionic concentrations. In particular, divalen ...
2023Real-time monitoring of single-cell secretion with a high-throughput nanoplasmonic microarray
Hatice Altug, Xiaokang Li, Yen-Cheng Liu, Saeid Ansaryan, Eduardo Romero Arvelo
Proteins secreted by cells play significant roles in mediating many physiological, developmental, and pathological processes due to their functions in intra/intercellular communication and signaling. Conventional end-point methods are insufficient for unde ...
2022Aptamer-modified biosensors to visualize neurotransmitter flux
Nako Nakatsuka, Emilie Vuille-Dit-Bille
Chemical biosensors with the capacity to continuously monitor various neurotransmitter dynamics can be powerful tools to understand complex signaling pathways in the brain. However, in vivo detection of neurochemicals is challenging for many reasons such a ...
2022Calcium-Mediated Liposome Fusion to Engineer Giant Lipid Vesicles with Cytosolic Proteins and Reconstituted Mammalian Proteins
Giant unilamellar lipid vesicles (GUVs) are widely used as model membrane systems and provide an excellent basis to construct artificial cells. To construct more sophisticated artificial cells, proteins-in particular membrane proteins-need to be incorporat ...
2020Photoregulated fluxional fluorophores for live-cell super-resolution microscopy with no apparent photobleaching
Photoswitchable mols. have multiple applications in the phys. and life sciences because their properties can be modulated with light. Fluxional mols., which undergo rapid degenerate rearrangements in the electronic ground state, also exhibit switching beha ...
Springer2019Estimating the Readily-Releasable Vesicle Pool Size at Synaptic Connections in the Neocortex
Henry Markram, Eilif Benjamin Muller, Srikanth Ramaswamy, Rodrigo de Campos Perin, Natali Barros Zulaica, Giuseppe Chindemi
Previous studies based on the 'Quantal Model' for synaptic transmission suggest that neurotransmitter release is mediated by a single release site at individual synaptic contacts in the neocortex. However, recent studies seem to contradict this hypothesis ...
2019ER-to-lysosome-associated degradation of proteasome-resistant ATZ polymers occurs via receptor-mediated vesicular transport
Maurizio Molinari, Paolo Paganetti
Maintenance of cellular proteostasis relies on efficient clearance of defective gene products. For misfolded secretory proteins, this involves dislocation from the endoplasmic reticulum (ER) into the cytosol followed by proteasomal degradation. However, po ...
2018