Concept

Point spread function

Related publications (101)

3-D PSF fitting for fluorescence microscopy: implementation and localization application

Michaël Unser, Daniel Sage, François Aguet, Hagay Kirshner

Localization microscopy relies on computationally efficient Gaussian approximations of the point spread function for the calculation of fluorophore positions. Theoretical predictions show that under specific experimental conditions, localization accuracy i ...

Wiley-Blackwell2013

Experimental study of z resolution in acousto-optical coherence tomography using random phase jumps on ultrasound and light

Salma Farahi

Acousto-optical coherence tomography (AOCT) is a variant of acousto-optic imaging (also called ultrasound modulated optical tomography) that makes possible to get resolution along the ultrasound propagation axis z. We present here AOCT experimental results ...

Optical Soc Amer2013

Marker-free phase nanoscopy

Pierre Magistretti, Christian Depeursinge, Nicolas Pavillon, Pierre Marquet, Pascal Jourdain, Daniel Boss, Muhammed Fatih Toy, Yann Jérôme Michel Pascal Cotte

We introduce a microscopic method that determines quantitative optical properties beyond the optical diffraction limit and allows direct imaging of unstained living biological specimens. In established holographic microscopy, complex fields are measured us ...

Nature Publishing Group2013

Fast Maximum Likelihood High-Density Low-SNR Super-Resolution Localization Microscopy

Michaël Unser, Kyungjin Kim

Localization microscopy such as STORM/PALM achieves the super-resolution by sparsely activating photo-switchable probes. However, to make the activation sparse enough to obtain reconstruction images using conventional algorithms, only small set of probes n ...

SampTA2013

Influence of reflecting walls on edge diffraction simulation in geometrical acoustics

Dirk Schröder

Edge diffraction can be introduced into Geometrical Acoustics mainly by three models: detour-based, energetic and wave-based diffraction models. In the past, we thoroughly compared Maekawa's detour law, the uncertainty relation based diffraction method and ...

Acoustical Society of America2013

Image analysis for cosmology: results from the GREAT10 Galaxy Challenge

Frédéric Courbin, Malte Tewes, Marc Gentile, Nicolas Cantale, Guldariya Nurbaeva, Richard Massey

In this paper, we present results from the weak-lensing shape measurement GRavitational lEnsing Accuracy Testing 2010 (GREAT10) Galaxy Challenge. This marks an order of magnitude step change in the level of scrutiny employed in weak-lensing shape measureme ...

Blackwell Publishing Ltd, Oxford2012

Reconstruction in interferometric synthetic aperture microscopy: comparison with optical coherence tomography and digital holographic microscopy

Christian Depeursinge, Shanshan Kou

It is shown that the spatial frequencies recorded in interferometric synthetic aperture microscopy do not correspond to exact backscattering [as they do in unistatic synthetic aperture radar (SAR)] and that the reconstruction process based on SAR is theref ...

2012

A fast empirical method for galaxy shape measurements in weak lensing surveys

Frédéric Courbin, Georges Meylan, Malte Tewes, Nicolas Cantale

We describe a simple and fast method to correct ellipticity measurements of galaxies from the distortion by the instrumental and atmospheric point spread function (PSF), in view of weak lensing shear measurements. The method performs a classification of ga ...

Edp Sciences S A2012

High-resolution cell outline segmentation and tracking from phase-contrast microscopy images

Jean-Jacques Meister, Charles Brepsant, Mark Etienne Ambühl

Accurate extraction of cell outlines from microscopy images is essential for analysing the dynamics of migrating cells. Phase-contrast microscopy is one of the most common and convenient imaging modalities for observing cell motility because it does not re ...

2012

A Psf-Based Approach To Biplane Calibration In 3D Super-Resolution Microscopy

Michaël Unser, Suliana Manley, Daniel Sage, Hagay Kirshner, Thomas Pengo

Super-resolution localization microscopy methods such as PALM and STORM have been shown to provide imaging with resolutions up to a few tens of nanometers while using relatively simple setups. Biplane PALM has extended the PALM technique to three-dimension ...

Ieee2012

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