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A method is presented to form gold-electrode-tethered lipid bilayers with exceptionally high elec. resistances. Elec. impedance spectroscopy is used to monitor the bilayer incorporation of a ligand-gated ion channel protein and the modulation of its channe ...
Changes in sample concentrations of CO2 or organic acids cause potential instabilities when polymer membranes are directly applied to the surface of ion-selective field-effect transistors (ISFETs). Currently used designs avoid this well-documented effect b ...
A generalized version of the integrate-and-fire model is presented that qualitatively reproduces firing rates and membrane trajectories of motoneurons. The description is based on the spike-response model and includes 3 different time constants: the passiv ...
Using both vibrational sum-frequency generation and fluorescence microscopy, the phase behaviour of a DPPC lipid monolayer on water is investigated as a function of surface pressure. The vibrational specificity of the sum-frequency generation techniques pe ...
Rhodopsin-transducin coupling was used as an assay to investigate a laterally patterned membrane reconstituted with a receptor and its G protein. It served as a model system to show the feasibility to immobilize G protein-coupled receptors on solid support ...
The atomic force microscope acquires topographs of single native membrane proteins at subnanometer resolution. Owing to the high signal-to-noise ratio, such images allow the conformational space of membrane protein surfaces to be sampled. This is demonstra ...
An X-ray diffraction experiment on multilamellar membranes incorporated into an X-ray waveguide structure is reported. In the device, the lipid bilayers are confined to one side by the silicon substrate and to the other side by an evaporated thin metal cap ...
While the existence of cholesterol/sphingolipid (raft) membrane domains in the plasma membrane is now supported by strong experimental evidence, the structure of these domains, their size, their dynamics, and their molecular composition remain to be unders ...
In this work the authors have shown that stable gigaohm seals over micrometer-sized holes can be obtained in a time-frame of seconds by the electrophoretic self-positioning of charged lipid membranes. For the first time, single-channel events were recorded ...
A new strategy for the reversible formation of supported lipid bilayers is introduced. It uses an extension of immobilized metal ion affinity chromatog. technol., which depends on the reversible formation of complexes between metal ion binding chelator gro ...