Concept
Bisulfite sequencing
Related publications (117)
Epigenome-wide DNA methylation in externalizing behaviours: A review and combined analysis
Maria del Carmen Sandi Perez, Mandy Meijer
DNA methylation (DNAm) is one of the most frequently studied epigenetic mechanisms facilitating the interplay of genomic and environmental factors, which can contribute to externalizing behaviours and related psychiatric disorders. Previous epigenome-wide ...
PERGAMON-ELSEVIER SCIENCE LTD2023DNA methylation sites in early adulthood characterised by pubertal timing and development: a twin study
Background Puberty is a highly heritable and variable trait, with environmental factors having a role in its eventual timing and development. Early and late pubertal onset are both associated with various diseases developing later in life, and epigenetic c ...
London2023Efficient and sensitive profiling of RNA–protein interactions using TLC-CLIP
Didier Trono, Julien Léonard Duc, Christina Ernst
RNA-binding proteins are instrumental for post-transcriptional gene regulation, controlling all aspects throughout the lifecycle of RNA molecules. However, transcriptome-wide methods to profile RNA-protein interactions in vivo remain technically challengin ...
2023Epigenetic ageing accelerates before antiretroviral therapy and decelerates after viral suppression in people with HIV in Switzerland: a longitudinal study over 17 years
Jacques Fellay, Christian Axel Wandall Thorball
Background Accelerated epigenetic ageing can occur in untreated HIV infection and is partially reversible with effective antiretroviral therapy (ART). We aimed to make a long-term comparison of epigenetic ageing dynamics in people with HIV during untreated ...
ELSEVIER2023Systematic benchmarking of single-cell ATAC-sequencing protocols
Bart Deplancke, Jörn Pezoldt, Camille Lucie Germaine Lambert
Benchmarking of scATAC-seq protocols demonstrates the effects of data quality on downstream analyses. Single-cell assay for transposase-accessible chromatin by sequencing (scATAC-seq) has emerged as a powerful tool for dissecting regulatory landscapes and ...
NATURE PORTFOLIO2023Natural competence of the pathogen Acinetobacter baumannii: an elusive phenomenon
Natural competence for transformation is an important driver of horizontal DNA exchange between different organisms. This can result in accumulation of dangerous genetic features, such as antibiotic resistance genes, in a single organism. One example of an ...
EPFL2022Method of preparation of cdna library useful for efficient mrna sequencing and uses thereof
Bart Deplancke, Riccardo Dainese, Daniel Alpern, Mustafa Demir
The invention relates to methods for the preparation of method of preparation of cDNA library based on one or many RNA samples useful for efficient RNA sequencing and uses thereof. The invention further relates to related tools and kits useful in said meth ...
2022Bubble-enhanced ultrasonic microfluidic chip for rapid DNA fragmentation
Martinus Gijs, Thomas Lehnert, Lin Sun
DNA fragmentation is an essential process in developing genetic sequencing strategies, genetic research, as well as for the diagnosis of diseases with a genetic signature like cancer. Efficient on-chip DNA fragmentation protocols would be beneficial to pro ...
ROYAL SOC CHEMISTRY2022Enrichment of gut microbiome strains for cultivation-free genome sequencing using droplet microfluidics
We report a droplet microfluidic method to target and sort individual cells directly from complex microbiome samples and to prepare these cells for bulk whole-genome sequencing without cultivation. We characterize this approach by recovering bacteria spike ...
2022The enhancement of DNA fragmentation in a bench top ultrasonic water bath with needle-induced air bubbles: Simulation and experimental investigation
Martinus Gijs, Thomas Lehnert, Yang Liu, Lin Sun
Shearing DNA to a certain size is the first step in many medical and biological applications, especially in next-generation gene sequencing technology. In this article, we introduced a highly efficient ultrasonic DNA fragmentation method enhanced by needle ...
AIP Publishing2022