Chaperones Effects: Amyloid Beta Peptide Aggregation

This lecture explores the contrasting effects of chaperones on amyloid beta peptide aggregation, investigating the kinetics and equilibrium of the process, the role of various chaperones like Brichos and scFv, and the impact on fibril mass concentration. The instructor discusses the search for secondary nucleation inhibitors, the use of isotopes to study oligomers, and the retardation of amyloid aggregation by chaperones like DNAJB6. Additionally, the lecture covers the quantification of oligomers using radio-isotopes, the binding of Brichos to fibrils, and the effects of antibodies on aggregation kinetics and binding. Various techniques such as SPR, MALDI, and immuno-gold EM are also presented.