Metabolism and Proteostasis Evolution

This lecture by Kausik Chakraborty explores the connection between metabolism and proteostasis through evolution, focusing on the intracellular homeostasis of protein biogenesis, the study of proteostasis capacity, and the role of mutational buffering in protein evolution. The lecture delves into how different osmolytes alter folding pathways and structures, the impact of genetic alterations in cellular metabolism on mutational buffering capacity, and the role of metabolites in altering protein folding. It also discusses the evolution of osmotolerant strains, the regulation of mutational buffering by the cellular chemical milieu, and the convergence of metabolite perturbation. The lecture concludes by highlighting the importance of small-molecule-dependent folding in reinforcing proteostasis.