Gene Editing and DNA Origami: Innovations in Biotechnology

This lecture covers the principles of recombinant DNA technology and its applications in gene editing, particularly focusing on CRISPR/Cas9. The instructor explains how recombinant DNA molecules are created through genetic recombination, allowing for the insertion of specific genes into plasmids. The process of DNA cloning is detailed, including the use of antibiotic resistance genes to select successfully modified bacteria. The lecture transitions to CRISPR/Cas9, highlighting its role as a bacterial immune system that can be harnessed for precise gene editing. The instructor discusses the mechanics of CRISPR, including the importance of guide RNA and the PAM sequence for targeting DNA. The lecture also introduces DNA origami, a technique for folding DNA into specific shapes for various applications, including drug delivery and nanotechnology. The potential of these technologies in medical advancements and ethical considerations surrounding gene editing are also addressed, emphasizing the transformative impact of DNA manipulation in biotechnology.