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This lecture delves into the existing methodologies for assessing life cycle impacts, exploring the differences in characterization models, algorithms, and regionalization. It covers factors like toxicity, ozone layer depletion, and climate change, comparing methodologies like IMPACT 2002+, ReCiPe 2016, and LIME. The instructor discusses the various impact categories, from human health to ecosystem quality, and the different approaches to damage assessment. Additionally, the lecture examines the temporal boundaries in impact assessment, focusing on greenhouse gases and their global warming potentials over different time frames.