This lecture introduces the immune system, focusing on physical barriers, restriction enzymes, and the defense mechanisms of bacteria. It covers the role of restriction enzymes in protecting bacterial DNA, their mechanism of action, and their applications in DNA manipulation. The lecture also discusses the nomenclature of restriction enzymes, examples of their cleavage sites, and their significance in genetic engineering. Furthermore, it explores the CRISPR-Cas system, detailing its components, mechanism, and applications in gene editing and disease treatment. The lecture concludes with a discussion on the ethical implications of gene editing and the future prospects of genetic modification.