Liquid Chromatography: Principles and Techniques

This lecture covers the principles and techniques of liquid chromatography, including different types such as affinity, HPLC, peak, ion, and size exclusion chromatography. It explains the role of elution, detectors, pumps, and columns in separating solutes based on their properties. The importance of solvent degassing, pressure control, and injection loops in maintaining system stability and accuracy is also discussed. Additionally, the lecture delves into the various detectors used in liquid chromatography, such as conductometric, refractometry, and spectrometry, highlighting their roles in detecting different types of solutes based on their properties.