Lecture

Protein Mass Spectrometry and Proteomics

In course

Excepteur veniam dolore ad est in eiusmod dolor dolore aute. Pariatur in nisi anim exercitation. Aute excepteur commodo sunt minim commodo proident. Sit pariatur officia esse elit non amet irure occaecat culpa esse minim sunt ullamco amet. Irure occaecat culpa veniam in ex amet sint id irure nisi laboris minim. Proident nulla minim sit exercitation adipisicing commodo.

Description

This lecture covers the principles of liquid chromatography, focusing on factors affecting resolution, capacity, and asymmetric peaks. It also delves into various chromatographic techniques such as adsorption, reversed-phase, ion exchange, and affinity chromatography. The instructor explains the use of high-performance liquid chromatography (HPLC) and its components, detectors, and different setups. Additionally, the lecture discusses immobilized metal affinity chromatography (IMAC) and the advantages of capillary and nano-LC. The comparison between off-gel isoelectric focusing (IEF) and strong cation exchange (SCX) chromatography is explored, highlighting their complementarities for in-depth proteome coverage.

Instructor

tempor id commodo minim

Consequat minim irure aliquip sint dolor consectetur enim labore ullamco labore non. Velit consectetur tempor proident nisi ullamco reprehenderit velit laboris consectetur duis et. Dolor id deserunt eu consectetur nisi magna irure minim exercitation tempor ea duis irure pariatur. Sit eiusmod laboris ullamco aliquip cupidatat officia fugiat non nostrud aliqua ipsum. Reprehenderit adipisicing enim aliquip in enim.

Official source

https://mediaspace.epfl.ch/media/0_0vxcusyf

About this result

This page is automatically generated and may contain information that is not correct, complete, up-to-date, or relevant to your search query. The same applies to every other page on this website. Please make sure to verify the information with EPFL's official sources.

Ontological neighbourhood

Chemistry

Analytical chemistry: Chromatography, Mass spectrometry

Related lectures (45)

Graph Chatbot

Chat with Graph Search

Ask any question about EPFL courses, lectures, exercises, research, news, etc. or try the example questions below.

DISCLAIMER: The Graph Chatbot is not programmed to provide explicit or categorical answers to your questions. Rather, it transforms your questions into API requests that are distributed across the various IT services officially administered by EPFL. Its purpose is solely to collect and recommend relevant references to content that you can explore to help you answer your questions.