Genetic Code Expansion & Applications in Fruit Fly

This lecture covers Genetic Code Expansion (GCE) and its applications in fruit fly models, focusing on encoding non-native amino acids with temporal control. It discusses the basics of the genetic code, translation machinery, and the Amber suppression method. The lecture explores the details of eukaryotic mRNA translation, the expansion of the genetic code, and the site-specific incorporation of nitro-tyrosine. It also delves into the powerful GAL4/UAS technique for targeted gene expression and the significance of Drosophila as a model organism. The lecture concludes with a study on proteome labeling and protein identification in specific tissues using GCE technology.