Protein Addressing at the Endoplasmic Reticulum

This lecture covers the process of protein addressing at the endoplasmic reticulum (ER), including the recognition of signal sequences, translocation mechanisms, and experimental methods to study protein topology. It explains how proteins are targeted to the ER, the role of ribosomes, and the distinction between soluble and transmembrane proteins. The instructor discusses the 'positive inside' rule, the concept of membrane protein topology, and the experimental validation of transmembrane segments. Additionally, the lecture explores the machinery involved in protein translocation, the structure of the translocon, and the different types of transmembrane proteins found in eukaryotic cells.