Flow Cytometry: An Invaluable Tool for Research and Clinical Applications

This lecture introduces Flow Cytometry as a technology for rapid multi-parametric analysis of single cells, using lasers to produce scattered and fluorescent light signals. It covers its applications in various disciplines such as immunology, stem cell biology, cancer biology, and infectious disease monitoring. The lecture explains the components of a Flow Cytometer, the process of labeling cells with fluorescent dyes, and the importance of compensation to correct spectral overlap in data analysis.