Altered Metabolism in Cancer: Warburg Effect and MYC Regulation

This lecture discusses the altered metabolism in cancer, focusing on the Warburg effect and the production of new metabolites associated with gene mutations like IDH1 and IDH2. It explores how MYC, a potent oncogene, controls various biological processes and the pathways frequently altered in cancer, such as the Rb and p53 pathways. The lecture also covers the re-activation of AKT by mTOR, the use of reporter constructs to understand transcription factor regulation, and techniques like FRET and yeast two-hybrid screens to analyze pathway activity and protein-protein interactions. Additionally, it delves into mass spectrometry analysis and mass cytometry for studying cell-specific protein interactions.