Protein Circuit Design

This lecture covers the design of protein-based processors for logic operations in living cells, including principles for protease-based logic gates and circuits. It explores examples of protease-based logic gates, analog-to-digital conversions, and dynamic control mechanisms. The lecture also delves into the applications of synthetic protein circuits in cell-based therapies, focusing on adoptive CAR T cell therapy and the limitations faced. Furthermore, it discusses engineering advanced logic and distributed computing in human CAR immune cells, highlighting the potential of protein circuits in providing useful capabilities for cell-based therapeutics.