Protein Circuit Design: Signal Transmission and Dynamic Control

This lecture explores protein-mediated signal transmission, focusing on de novo interactions and engineered scaffold systems. It discusses how natural and engineered circuits route signals, the analysis of diverter scaffold requirements, and the rewiring of scaffold-mediated information transmission. The instructor also covers protein-based processors performing logic operations, protease-based logic gates, and analog-to-digital conversions. Additionally, it delves into protein-based dynamic control systems, including negative feedback, oscillation, multistability, and memory. The lecture concludes with applications of synthetic protein circuits in engineered cell-based therapies, specifically adoptive CAR T cell therapy, its limitations, and the potential of protein circuits in cell-based therapeutics.