Lecture

Protein Mass Spectrometry Insights

Description

This lecture delves into the mechanisms of material ejection in MALDI mass spectrometry, exploring the influence of laser fluence and spot size on ejection mechanisms and plume development. It discusses the complex cascade of events in MALDI, emphasizing the importance of laser parameters and analyte-matrix properties. The presentation provides insights into the unexplained spot size effect in MALDI, shedding light on analyte fragmentation and plume dynamics. Additionally, it covers mass analyzers in proteomics, including Quadrupole, Orbitrap, and Time of Flight, explaining their principles and applications.

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