Lecture
This lecture covers the analysis of intensity and localization in a dataset of images with three fluorescent widefield channels acquired for three conditions (Wells 06, 08, 11). The microscope used outputs individual tiff images representing DAPI staining, Phalloidin (AlexaF488), and a Red Fluorescent protein fused to the protein of interest. The main goal is to measure the change in the expression of the protein of interest across different conditions.