Microscopy Image Analysis

This lecture covers the principles of light microscopy, focusing on the use of fluorescent proteins for cellular imaging. It delves into the concepts of GFP/RFP mutations, applications of fluorescent proteins, optical slicing methods, and modulation transfer functions. The instructor discusses the challenges in imaging thick specimens, the benefits of timelapse microscopy, and the techniques for signal and sampling in microscopy. Moreover, the lecture explores the advancements in spinning disk confocal microscopy and the resolution limits in light microscopy.