Eukaryotic Transcription: Initiation and Elongation

This lecture covers the process of eukaryotic transcription, focusing on the initiation and elongation phases. Topics include the differences between bacterial and eukaryotic transcription, the maturation of RNA, the addition of the 5' cap, splicing, and the role of the mediator complex. The instructor explains the mechanisms of transcription initiation step by step, the importance of factors like TBP and TFIIH, and the phosphorylation of the CTD domain. Additionally, the lecture delves into the regulation of transcription in eukaryotes, discussing activators, repressors, and the combinatorial control of gene expression through regulatory proteins. The session concludes with insights into heterodimerization of transcription factors and the complexity of gene regulation.