Light Sources: Metal Halide Lamps & LEDs

This lecture covers the operation and features of metal halide lamps, which produce light by ionizing gases in an electric arc. It also discusses the anatomy of metal halide lamps and their use in microscopy. Additionally, it explores the technology and advantages of Light Emitting Diodes (LEDs) in microscopy imaging, highlighting their efficiency, spectrum, and power. The lecture further delves into the properties of lasers as light sources for fluorescence microscopy, including their high intensity, uniform wavelength, and different types for broad spectral coverage. It concludes by explaining the applications of lasers in advanced microscopy techniques like confocal imaging and super-resolution microscopy.