Fluorescence Microscopy: Illumination and Sources

This lecture covers the basic principles of fluorescence microscopy, including the properties of fluorescent dyes, different types of fluorescence markers, and the optical components required for proper imaging. It explains the importance of proper illumination in microscopy, detailing the selection of wavelength and intensity of the light source, as well as the alignment and focusing techniques. Various illumination sources such as incandescent lamps, quartz tungsten-halogen, lon arc lamps, and solid-state sources like LEDs and lasers are discussed. The lecture also delves into the spectrum of incandescent lamps, control of light using reflectors, and the operation of lon arc lamps in microscopy.