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This lecture discusses the nonequilibrium equality for free energy differences proposed by Crooks and Jarzynski in 1997. The lecture covers the entropy production fluctuation theorem, nonequilibrium work relation, and the difference in free energy between folded and unfolded states. It explores the concept of irreversible processes, heat generation, and the Clasius relation. The presentation delves into the theoretical framework behind the equality, providing insights into the reversible and irreversible nature of processes. The lecture concludes by emphasizing the significance of work done in different states and the extraction of work in a system.