Programmable RNA-Targeting & Genome Editing

This lecture covers the development and applications of programmable RNA-targeting tools, focusing on CRISPR-cas systems. It delves into the history of CRISPR, its adaptive immune system in prokaryotes, and the mechanisms of CRISPR-cas9 gene editing. The lecture also explores strategies for base editing in eukaryotic genomes, the importance of PAM motifs, and the use of Cas13 and Cas12 orthologs for CRISPR diagnostics. Additionally, it discusses the methodology for programmable RNA targeting, protein-based RNA effectors, and detection strategies for CoVID-19, including the SHERLOCK detection assay.