Genome Editing: CRISPR-Cas Applications

This lecture explores the applications of CRISPR-Cas in genome editing, focusing on the engineering of bacterial genomes to store information, curing genetic diseases through zinc finger nucleases, TAL effector nucleases, and CRISPR-Cas systems. The lecture delves into the simplicity and multiplexing capabilities of guide RNA, the specificity and challenges of Cas9, and the potential of genome editing in curing diseases like sickle cell anemia. It also covers the mechanisms of DNA damage, knockout strategies, and the use of HDR to rescue diseases. The lecture concludes with discussions on base editors, prime editing, and the future prospects of genome editing technologies.