Single Cell mRNA Sequencing

This lecture covers the Drop Seq method for single-cell mRNA sequencing, which involves barcoded beads with unique primers to amplify mRNA from individual cells, creating droplets with cells and beads for sequencing. The process includes cell lysis, mRNA hybridization, cell barcode creation, and library generation. Additionally, it compares RNA Microarray with RNA sequencing, highlighting the differences in detection, quantification, and data processing requirements.