DNA Sequencing: Sanger and Illumina Methods

This lecture covers the principles of Sanger sequencing, including the use of ddNTP terminators and electrophoresis, as well as the limitations of sequencing one fragment at a time. It also explores the advancements in newer methods such as real-time sequencing and massive parallel sequencing using platforms like HiSeq and MiSeq. Additionally, the lecture delves into Illumina sequencing, detailing the process of cluster formation and sequencing on a flow cell.