Lecture
DNA Sequencing: Sanger and Illumina Methods
Description
This lecture covers the principles of Sanger sequencing, including the use of ddNTP terminators and electrophoresis, as well as the limitations of sequencing one fragment at a time. It also explores the advancements in newer methods such as real-time sequencing and massive parallel sequencing using platforms like HiSeq and MiSeq. Additionally, the lecture delves into Illumina sequencing, detailing the process of cluster formation and sequencing on a flow cell.
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