Microscopy and Imaging Systems

This lecture introduces the fundamental concepts of microscopy and imaging systems, explaining the need for microscopes to magnify objects beyond the capabilities of the human eye. It covers topics such as the functioning of lenses, the resolving power of the eye, the use of magnifying glasses, compound microscopes, infinity corrected microscopes, microscope objectives, illumination systems, fluorescence microscopy, and super resolution techniques. The lecture also delves into the principles of resolution, the role of structured illumination in microscopy, and advanced techniques like STED microscopy. Various microscopy modes, including bright-field, dark-field, and phase contrast, are discussed, highlighting the importance of objective quality and illumination techniques.