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This lecture explores the impact of sex differences on immunity, focusing on the role of hormones, microbiome, and age in shaping immune responses. It discusses how hormones like progesterone and estrogens influence immune cell reactivity, the microbiome's regulation of autoimmunity, and the varying inflammatory responses at different life stages. The presentation emphasizes the importance of considering both sexes in immune function studies to understand the complex interactions between hormones, genes, and the environment.